Fluorescence microscopy provides an efficient and unique approach to study fixed and
living cells because of its versatility, specificity, and high sensitivity. Fluorescence …

Single-molecule fluorescence spectroscopy and super-resolution microscopy are important
elements of the ongoing technical revolution to reveal biochemical and cellular processes in …

Decoding cellular processes requires visualization of the spatial distribution and dynamic
interactions of biomolecules. It is therefore not surprising that innovations in imaging …

The alternating-laser excitation (ALEX) scheme continues to expand the possibilities of
fluorescence-based assays to study biological entities and interactions. Especially the …

In recent years, a number of approaches have emerged that enable far‐field fluorescence
imaging beyond the diffraction limit of light, namely super‐resolution microscopy. These …

We synthesized five fluorophore–photochrome dyads designed to switch reversibly between
nonfluorescent and fluorescent isomers under optical control. These compounds pair an …

Sub-diffraction microscopy enables bio-imaging with unprecedented clarity. However, most
super-resolution methods require complex, costly purpose-built systems, involve image post …

Photoactivatable fluorophores switch from a nonemissive state to an emissive one under
irradiation at an activation wavelength and then emit light in the form of fluorescence upon …

Increasing demand for detecting single molecules in challenging environments has raised
the bar for the fluorophores used. To achieve better resolution and/or contrast in …

Modern fluorescence microscopy applications go along with increasing demands for the
employed fluorescent dyes. In this work, we compared antifading formulae utilizing a …