During aerobic growth on glucose, Escherichia coli excretes acetate, a mechanism called
“overflow metabolism.” At high concentrations, the secreted acetate inhibits growth. Several …

Gene expression is controlled by the joint effect of (i) the global physiological state of the
cell, in particular the activity of the gene expression machinery, and (ii) DNA‐binding …

Fluorescent reporter proteins based on flavin-binding photosensors were recently
developed as a new class of genetically encoded probes characterized by small size and …

New microbes are being engineered that contain the genetic circuitry, metabolic pathways,
and other cellular functions required for a wide range of applications such as producing …

The ability to control growth is essential for fundamental studies of bacterial physiology and
biotechnological applications. We have engineered an Escherichia coli strain in which the …

Escherichia coli adapts its lifestyle to the variations of environmental growth conditions,
swap** between swimming motility or biofilm formation. The stationary-phase sigma factor …

For an industrial fermentation process, it can be advantageous to decouple cell growth from
product formation. This decoupling would allow for the rapid accumulation of biomass …

Type II toxin-antitoxin (TA) systems are classically composed of two genes that encode a
toxic protein and a cognate antitoxin protein. Both genes are organized in an operon whose …

Accurate characterization of promoter behavior is essential for the rational design of
functional synthetic transcription networks such as logic gates and oscillators. However …

Background To connect gene expression with cellular physiology, we need to follow levels
of proteins over time. Experiments typically use variants of Green Fluorescent Protein (GFP) …