In the last decade, single-cell RNA sequencing routinely performed on large numbers of
single cells has greatly advanced our understanding of the underlying heterogeneity of …

Mass spectrometry (MS)-based proteomics has become a powerful technology to quantify
the entire complement of proteins in cells or tissues. Here, we review challenges and recent …

Analyzing proteins from single cells by tandem mass spectrometry (MS) has recently
become technically feasible. While such analysis has the potential to accurately quantify …

Single-cell resolution analysis of complex biological tissues is fundamental to capture cell-
state heterogeneity and distinct cellular signaling patterns that remain obscured with …

Major aims of single-cell proteomics include increasing the consistency, sensitivity and
depth of protein quantification, especially for proteins and modifications of biological interest …

Mass spectrometry is unmatched in its versatility for studying practically any aspect of the
proteome. Because the foundations of mass spectrometry-based proteomics are complex …

Mammalian cells have about 30,000 times as many protein molecules as mRNA molecules,
which has major implications in the development of proteomics technologies. We discuss …

Single-cell proteomics by mass spectrometry is emerging as a powerful and unbiased
method for the characterization of biological heterogeneity. So far, it has been limited to …

The recent technological and computational advances in mass spectrometry-based single-
cell proteomics have pushed the boundaries of sensitivity and throughput. However …

Recent technological advances in mass spectrometry promise to add single-cell proteomics
to the biologist's toolbox. Here we discuss the current status and what is needed for this …