Droplet digital PCR as an emerging tool in detecting pathogens nucleic acids in infectious diseases

B Chen, Y Jiang, X Cao, C Liu, N Zhang, D Shi - Clinica Chimica Acta, 2021 - Elsevier
The microbial infectious diseases (infectious diseases) represent the leading global public
health problem, and the effective treatment depends on rapid and accurate detection of …

Advances in the diagnosis of foot-and-mouth disease

CL Wong, CY Yong, HK Ong, KL Ho… - Frontiers in veterinary …, 2020 - frontiersin.org
Foot-and-mouth disease (FMD) is a devastating livestock disease caused by foot-and-mouth
disease virus (FMDV). Outbreaks of this disease in a country always result in conspicuous …

Changes in peripheral blood cytokines in patients with severe fever with thrombocytopenia syndrome

Z He, B Wang, Y Li, K Hu, Z Yi, H Ma… - Journal of Medical …, 2021 - Wiley Online Library
Severe fever with thrombocytopenia syndrome (SFTS) is recognized as an emerging
infectious disease. This study aimed to investigate the pathogenic mechanism of SFTS. A …

Development and validation of a droplet digital PCR assay for Nipah virus quantitation

J Shuai, K Chen, X Han, R Zeng, H Song, L Fu… - BMC Veterinary …, 2024 - Springer
Abstract Background Nipah virus (NiV) is a zoonotic pathogen that poses a significant threat
because of its wide host range, multiple transmission modes, high transmissibility, and high …

[HTML][HTML] Competitiveness of quantitative polymerase chain reaction (qPCR) and droplet digital polymerase chain reaction (ddPCR) technologies, with a particular …

S Park, A Rana, W Sung, M Munir - Applied Microbiology, 2021 - mdpi.com
With fast-growing polymerase chain reaction (PCR) technologies and various application
methods, the technique has benefited science and medical fields. While having strengths …

[HTML][HTML] SARS-CoV-2 RNA quantification using droplet digital RT-PCR

NN Kinloch, G Ritchie, W Dong, KD Cobarrubias… - The Journal of Molecular …, 2021 - Elsevier
Quantitative viral load assays have transformed our understanding of viral diseases. They
hold similar potential to advance COVID-19 control and prevention, but SARS-CoV-2 viral …

Development of a droplet digital PCR assay to detect bovine alphaherpesvirus 1 in bovine semen

Z Yu, Z Zhao, L Chen, H Yan, Q Cui, X Ju… - BMC Veterinary …, 2022 - Springer
Background Infectious bovine rhinotracheitis (IBR) caused by bovine alphaherpesvirus 1
(BoHV-1) is one of the most important contagious diseases in bovine. This is one of the most …

Reverse transcriptase droplet digital PCR to identify the emerging vesicular virus Senecavirus A in biological samples

TF Pinheiro‐de‐Oliveira… - Transboundary and …, 2019 - Wiley Online Library
Senecavirus A (SVA) belonging to the family Picornaviridae, genus Senecavirus was
incidentally isolated in 2002 from the PER. C6 (transformed foetal retinoblast) cell line …

Development and application of a sensitive droplet digital PCR (ddPCR) for the detection of infectious spleen and kidney necrosis virus

Q Lin, X Fu, L Liu, H Liang, Y Niu, Y Wen, Z Huang… - Aquaculture, 2020 - Elsevier
Infectious spleen and kidney necrosis, caused by the infectious spleen and kidney necrosis
virus (ISKNV), has damaged the economy of the world fish industry. Droplet digital PCR …

Development of quantitative PCR and digital PCR for the quantification of Leishmania infantum in dogs

DCA Pereira, RG Teixeira-Neto, VV Lopes… - Molecular and Cellular …, 2023 - Springer
Leishmaniasis is a zoonotic disease with worldwide distribution. In the Americas, the
causative agent of the visceral form is the protozoa Leishmania (Leishmania) infantum …