While often obvious for macroscopic organisms, determining whether a microbe is dead or
alive is fraught with complications. Fields such as microbial ecology, environmental health …

Abstract The World Health Organization (WHO) has identified the spread of antibiotic
resistance as one of the major risks to global public health. An important transfer route into …

Extracellular DNA from dead microorganisms can persist in soil for weeks to years 1–3.
Although it is implicitly assumed that the microbial DNA recovered from soil predominantly …

Nucleic acid-based analytical methods, ranging from species-targeted PCRs to
metagenomics, have greatly expanded our understanding of microbiological diversity in …

The lung microbiome, which is believed to be stable or at least transient in healthy people, is
now considered as a poly-microorganism component contributing to disease pathogenesis …

The limitation of 16S rRNA gene sequencing (DNA-based) for microbial community
analyses in water is the inability to differentiate live (dormant cells as well as growing or non …

Assessing bacterial contamination in environmental samples is critical in determining threats
to public health. The classical methods are time-consuming and only recognize species that …

Abstract Background The International Space Station (ISS) is a unique built environment
due to the effects of microgravity, space radiation, elevated carbon dioxide levels, and …

Aquatic environments harbour large and diverse microbial populations that ensure their
functioning and sustainability. In the current context of global change, characterizing …

Biological stability of drinking water implies that the concentration of bacterial cells and
composition of the microbial community should not change during distribution. In this study …