STED nanoscopy: a glimpse into the future

P Bianchini, C Peres, M Oneto, S Galiani… - Cell and tissue …, 2015 - Springer
The well-known saying of “Seeing is believing” became even more apt in biology when
stimulated emission depletion (STED) nanoscopy was introduced in 1994 by the Nobel …

An evaluation of two‐photon excitation versus confocal and digital deconvolution fluorescence microscopy imaging in Xenopus morphogenesis

A Periasamy, P Skoglund, C Noakes… - … research and technique, 1999 - Wiley Online Library
The ability to visualize cell motility occurring deep in the context of opaque tissues will allow
many currently intractable issues in developmental biology and organogenesis to be …

Probing cytoskeletal structures by coupling optical superresolution and AFM techniques for a correlative approach

JV Chacko, FC Zanacchi, A Diaspro - Cytoskeleton, 2013 - Wiley Online Library
In this article, we describe and show the application of some of the most advanced
fluorescence superresolution techniques, STED AFM and STORM AFM microscopy towards …

Simultaneous multiplane confocal microscopy using acoustic tunable lenses

M Duocastella, G Vicidomini, A Diaspro - Optics express, 2014 - opg.optica.org
Maximizing the amount of spatiotemporal information retrieved in confocal laser scanning
microscopy is crucial to understand fundamental three-dimensional (3D) dynamic processes …

Some trends in microscope image processing

N Bonnet - Micron, 2004 - Elsevier
The present review tries to identify some trends among the multitude of ways followed by
image processing developments in the field of microscopy. Nine topics were selected. They …

A new method for blood cell image segmentation and counting based on PCNN and autowave

S Mao-jun, W Zhao-bin, Z Hong-juan… - 2008 3rd International …, 2008 - ieeexplore.ieee.org
In the field of biomedicine, because of cells' complex nature, it still remains a challenging
task to segment cells from its background and count them automatically. The Pulse-Coupled …

Adapting a compact confocal microscope system to a two‐photon excitation fluorescence imaging architecture

A Diaspro, M Corosu, P Ramoino… - … research and technique, 1999 - Wiley Online Library
Within the framework of a national National Institute of Physics of Matter (INFM) project, we
have realised a two‐photon excitation (TPE) fluorescence microscope based on a new …

[PDF][PDF] A counting and segmentation method of blood cell image with logical and morphological feature of cell

YD Ma, R Dai, L Li - Chinese Journal of Electronics, 2002 - researchgate.net
A new, simple method of counting and segmenting cell image is suggested in this paper. It is
based on the feature of cell's logical and morphological information. By using of …

A single-pinhole confocal laser scanning microscope for 3-D imaging of biostructures

A Diaspro, S Annunziata, M Raimondo… - IEEE Engineering in …, 1999 - ieeexplore.ieee.org
Confocal laser scanning microscopy (CLSM) is a powerful three-dimensional (3-D) tool at
the microscopic level, both for functional and for structural studies. The confocal microscope …

Time‐variant analysis of organelle and vesicle movement during phagocytosis in Paramecium primaurelia by means of fluorescence confocal laser scanning …

P Ramoino, F Beltrame, A Diaspro… - … research and technique, 1996 - Wiley Online Library
Vital fluorescent dyes (FITC‐albumin, Texas Red‐albumin, and acridine orange) were used
together with a confocal laser scanning optical microscope (CLSM) to display and analyze …