A transcriptome constructed from short-read RNA sequencing (RNA-seq) is an easily
attainable proxy catalog of protein-coding genes when genome assembly is unnecessary …

By exerting pro-and anti-tumorigenic actions, tumor-infiltrating immune cells can profoundly
influence tumor progression, as well as the success of anti-cancer therapies. Therefore, the …

We introduce quanTIseq, a method to quantify the fractions of ten immune cell types from
bulk RNA-sequencing data. quanTIseq was extensively validated in blood and tumor …

Advances in DNA sequencing technology have revolutionized the field of molecular analysis
of trophic interactions, and it is now possible to recover counts of food DNA sequences from …

RNA-seq is currently considered the most powerful, robust and adaptable technique for
measuring gene expression and transcription activation at genome-wide level. As the …

Differential gene expression (DGE) analysis is one of the most common applications of RNA-
sequencing (RNA-seq) data. This process allows for the elucidation of differentially …

Whole transcriptome analysis plays an essential role in deciphering genome structure and
function, identifying genetic networks underlying cellular, physiological, biochemical and …

Abstract EnTAP (Eukaryotic Non‐Model Transcriptome Annotation Pipeline) was designed
to improve the accuracy, speed, and flexibility of functional gene annotation for de novo …

Statistical analyses are a crucial component of the biomedical research process and are
necessary to draw inferences from biomedical research data. The application of sound …

The human microbiota is a complex ecological community of commensal, symbiotic and
pathogenic microorganisms harboured by the human body. Next-generation sequencing …