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Pinpointing Fat Molecules: Advances in Coherent Raman Scattering Microscopy for Lipid Metabolism
At the heart of numerous diseases, lipids are a diverse array of chemically and functionally
heterogeneous compounds found in organisms. Their significance as energy sources …
heterogeneous compounds found in organisms. Their significance as energy sources …
Profiling single cancer cell metabolism via high-content SRS imaging with chemical sparsity
Metabolic reprogramming in a subpopulation of cancer cells is a hallmark of tumor
chemoresistance. However, single-cell metabolic profiling is difficult because of the lack of a …
chemoresistance. However, single-cell metabolic profiling is difficult because of the lack of a …
Background signals in stimulated Raman scattering microscopy and current solutions to avoid them
L Genchi, SP Laptenok, C Liberale - Advances in Physics: X, 2023 - Taylor & Francis
Stimulated Raman scattering (SRS) microscopy has gained popularity in recent years due to
its linearity to molecule concentration and laser intensity, and to the lack of the nonresonant …
its linearity to molecule concentration and laser intensity, and to the lack of the nonresonant …
Full-spectrum CARS microscopy of cells and tissues with ultrashort white-light continuum pulses
F Vernuccio, R Vanna, C Ceconello… - The Journal of …, 2023 - ACS Publications
Coherent anti-Stokes Raman scattering (CARS) microscopy is an emerging nonlinear
vibrational imaging technique that delivers label-free chemical maps of cells and tissues. In …
vibrational imaging technique that delivers label-free chemical maps of cells and tissues. In …
Fingerprint multiplex CARS at high speed based on supercontinuum generation in bulk media and deep learning spectral denoising
F Vernuccio, A Bresci, B Talone, A de la Cadena… - Optics …, 2022 - opg.optica.org
We introduce a broadband coherent anti-Stokes Raman scattering (CARS) microscope
based on a 2-MHz repetition rate ytterbium laser generating 1035-nm high-energy (≈ µJ …
based on a 2-MHz repetition rate ytterbium laser generating 1035-nm high-energy (≈ µJ …
Nonlinear imaging histopathology: a pipeline to correlate gold-standard hematoxylin and eosin staining with modern nonlinear microscopy
KF Tehrani, J Park, EJ Chaney, H Tu… - IEEE Journal of …, 2023 - ieeexplore.ieee.org
Hematoxylin and eosin (H&E) staining, the century-old technique, has been the gold
standard tool for pathologists to detect anomalies in tissues and diseases such as cancer …
standard tool for pathologists to detect anomalies in tissues and diseases such as cancer …
Simultaneous label-free autofluorescence multi-harmonic microscopy driven by the supercontinuum generated from a bulk nonlinear crystal
A De la Cadena, J Park, KF Tehrani… - Biomedical Optics …, 2024 - opg.optica.org
Nonlinear microscopy encompasses several imaging techniques that leverage laser
technology to probe intrinsic molecules of biological specimens. These native molecules …
technology to probe intrinsic molecules of biological specimens. These native molecules …
[HTML][HTML] Coherent Raman spectroscopy: quo vadis?
K Brzozowski, W Korona, A Nowakowska… - Vibrational …, 2024 - Elsevier
Although the potential of Coherent Raman Spectroscopy (CRS) in the area of biomedicine,
life sciences and material sciences has been well demonstrated, its wide-spread practical …
life sciences and material sciences has been well demonstrated, its wide-spread practical …
Label-free multimodal nonlinear optical microscopy reveals features of bone composition in pathophysiological conditions
B Talone, A Bresci, F Manetti, F Vernuccio… - … in Bioengineering and …, 2022 - frontiersin.org
Bone tissue features a complex microarchitecture and biomolecular composition, which
determine biomechanical properties. In addition to state-of-the-art technologies, innovative …
determine biomechanical properties. In addition to state-of-the-art technologies, innovative …
[HTML][HTML] Dual picosecond fast tunable optical parametric amplifier laser system for wide-field nonlinear optical microscopy
F Vernuccio, A Benachir, EM Fantuzzi, B Morel, S Bux… - APL Photonics, 2024 - pubs.aip.org
In the last decades, nonlinear optical microscopy techniques, such as two-Photon Excited
Fluorescence (2PEF), Sum Frequency Generation (SFG), and Hyperspectral Coherent Anti …
Fluorescence (2PEF), Sum Frequency Generation (SFG), and Hyperspectral Coherent Anti …