Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy
Single-molecule force spectroscopy has emerged as a powerful tool to investigate the forces
and motions associated with biological molecules and enzymatic activity. The most common …
and motions associated with biological molecules and enzymatic activity. The most common …
Chromosome replication and segregation in bacteria
In dividing cells, chromosome duplication once per generation must be coordinated with
faithful segregation of newly replicated chromosomes and with cell growth and division …
faithful segregation of newly replicated chromosomes and with cell growth and division …
Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs
Deep sequencing technologies such as Illumina, SOLiD, and 454 platforms have become
very powerful tools in discovering and quantifying small RNAs in diverse organisms …
very powerful tools in discovering and quantifying small RNAs in diverse organisms …
The hexameric helicase DnaB adopts a nonplanar conformation during translocation
O Itsathitphaisarn, RA Wing, WK Eliason, J Wang… - Cell, 2012 - cell.com
DNA polymerases can only synthesize nascent DNA from single-stranded DNA (ssDNA)
templates. In bacteria, the unwinding of parental duplex DNA is carried out by the replicative …
templates. In bacteria, the unwinding of parental duplex DNA is carried out by the replicative …
Single-molecule visualization of fast polymerase turnover in the bacterial replisome
The Escherichia coli DNA replication machinery has been used as a road map to uncover
design rules that enable DNA duplication with high efficiency and fidelity. Although the …
design rules that enable DNA duplication with high efficiency and fidelity. Although the …
Revealing the competition between peeled-Ssdna, melting bubbles and S-DNA during DNA overstretching using fluorescence microscopy
By using a dual laser optical tweezers with a fast force feedback, we were able to record the
exponential elongation following force steps imposed on D-phage ds-DNA molecule and on …
exponential elongation following force steps imposed on D-phage ds-DNA molecule and on …
[PDF][PDF] Single-molecule characterization of SV40 replisome and novel factors: human FPC and Mcm10
Y Ouyang, A Al-Amodi, M Tehseen… - Nucleic Acids …, 2024 - academic.oup.com
Abstract The simian virus 40 (SV40) replisome only encodes for its helicase; large T-antigen
(L-Tag), while relying on the host for the remaining proteins, making it an intriguing model …
(L-Tag), while relying on the host for the remaining proteins, making it an intriguing model …
Embracing Heterogeneity: Challenging the Paradigm of Replisomes as Deterministic Machines
The paradigm of cellular systems as deterministic machines has long guided our
understanding of biology. Advancements in technology and methodology, however, have …
understanding of biology. Advancements in technology and methodology, however, have …
Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target
New antibiotics with novel modes of action are required to combat the growing threat posed
by multi-drug resistant bacteria. Over the last decade, genome sequencing and other high …
by multi-drug resistant bacteria. Over the last decade, genome sequencing and other high …
Motors, switches, and contacts in the replisome
SM Hamdan, CC Richardson - Annual review of biochemistry, 2009 - annualreviews.org
Replisomes are the protein assemblies that replicate DNA. They function as molecular
motors to catalyze template-mediated polymerization of nucleotides, unwinding of DNA, the …
motors to catalyze template-mediated polymerization of nucleotides, unwinding of DNA, the …