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[HTML][HTML] Latest developed strategies to minimize the off-target effects in CRISPR-Cas-mediated genome editing
Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …
Patterns of CRISPR/Cas9 activity in plants, animals and microbes
The CRISPR/Cas9 system and related RNA‐guided endonucleases can introduce double‐
strand breaks (DSBs) at specific sites in the genome, allowing the generation of targeted …
strand breaks (DSBs) at specific sites in the genome, allowing the generation of targeted …
Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells
The activities and genome-wide specificities of CRISPR-Cas Cpf1 nucleases are not well
defined. We show that two Cpf1 nucleases from Acidaminococcus sp. BV3L6 and …
defined. We show that two Cpf1 nucleases from Acidaminococcus sp. BV3L6 and …
Multiplex gene regulation by CRISPR-ddCpf1
The clustered regularly interspaced short palindromic repeats (CRISPR)/dCas9 system has
been widely applied in both transcriptional regulation and epigenetic studies. However, for …
been widely applied in both transcriptional regulation and epigenetic studies. However, for …
Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage
Cpf1 is an RNA-guided endonuclease that is emerging as a powerful genome-editing tool,.
Here we provide insight into its DNA-targeting mechanism by determining the structure of …
Here we provide insight into its DNA-targeting mechanism by determining the structure of …
Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing
CRISPR‐Cas9 and Cas12a are two powerful genome editing systems. Expression of
CRISPR in plants is typically achieved with a mixed dual promoter system, in which Cas …
CRISPR in plants is typically achieved with a mixed dual promoter system, in which Cas …
CRISPR-Cas12a induced DNA double-strand breaks are repaired by multiple pathways with different mutation profiles in Magnaporthe oryzae
CRISPR-Cas mediated genome engineering has revolutionized functional genomics.
However, understanding of DNA repair following Cas-mediated DNA cleavage remains …
However, understanding of DNA repair following Cas-mediated DNA cleavage remains …
CRISPR/Cas-based genome engineering in natural product discovery
Covering: up to February, 2018 This review briefly introduces and summarizes current
knowledge about the Clustered Regularly Interspaced Short Palindromic Repeats …
knowledge about the Clustered Regularly Interspaced Short Palindromic Repeats …
Engineering introns to express RNA guides for Cas9-and Cpf1-mediated multiplex genome editing
D Ding, K Chen, Y Chen, H Li, K **e - Molecular plant, 2018 - cell.com
The clustered regularly interspaced short palindromic repeat (CRISPR) system has emerged
as the revolutionary platform for DNA targeting. This system uses a site-specific RNA guide …
as the revolutionary platform for DNA targeting. This system uses a site-specific RNA guide …
A 'new lease of life': FnCpf1 possesses DNA cleavage activity for genome editing in human cells
M Tu, L Lin, Y Cheng, X He, H Sun, H **e… - Nucleic acids …, 2017 - academic.oup.com
Cpf1 nucleases were recently reported to be highly specific and programmable nucleases
with efficiencies comparable to those of SpCas9. AsCpf1 and LbCpf1 require a single crRNA …
with efficiencies comparable to those of SpCas9. AsCpf1 and LbCpf1 require a single crRNA …