LUMA (LUminometric Methylation Assay)—a high throughput method to the analysis of genomic DNA methylation

M Karimi, S Johansson, D Stach, M Corcoran… - Experimental cell …, 2006 - Elsevier
Changes in genomic DNA methylation are recognized as important events in normal and
pathological cellular processes, contributing both to normal development and differentiation …

A new technique for obtaining whole pathogen transcriptomes from infected host tissues

TL Azhikina, TA Skvortsov, TV Radaeva… - …, 2010 - Taylor & Francis
We propose a novel experimental approach based on coincidence cloning for analyzing
sequences of bacterial intracellular pathogens specifically transcribed in affected tissues. Co …

Global DNA methylation analysis using the luminometric methylation assay

M Karimi, K Luttropp, TJ Ekström - Epigenetics Protocols, 2011 - Springer
Epigenetic alterations regulate the utilization of the genome by permitting or inhibiting
access of transcription factors and associated complexes. Although there are several …

Epigenetics—a helpful tool to better understand processes in clinical nephrology?

P Stenvinkel, TJ Ekström - Nephrology Dialysis Transplantation, 2008 - academic.oup.com
Epigenetics (literally in addition to the genetic sequence) is a novel discipline that has
languished in the shadow of its genomic big brother that has attracted little interest among …

Study of tissue-specific CpG methylation of DNA in extended genomic loci

TL Azhikina, ED Sverdlov - Biochemistry (Moscow), 2005 - Springer
Modern approaches for studies on genome functioning include investigation of its epigenetic
regulation. Methylation of cytosines in CpG dinucleotides is an inherited epigenetic …

A review of the transcriptome analysis of bacterial pathogens in vivo: Problems and solutions

TA Skvortsov, TL Azhikina - Russian Journal of Bioorganic Chemistry, 2010 - Springer
This review is devoted to the state-of-the-art strategies for the in vivo whole-transcriptome
analysis of intracellular pathogens. The methods for the initial enrichment with bacterial RNA …

Two-dimensional electrophoretic mobility shift assay: Identification and map** of transcription factor CTCF target sequences within an FXYD5–COX7A1 region of …

AS Vetchinova, SB Akopov, IP Chernov… - Analytical …, 2006 - Elsevier
An approach for fast identification and map** of transcription factor binding sites within
long genomic sequences is proposed. Using this approach, 10 CCCTC-binding factor …

Global analysis of DNA 5-methylcytosine using the luminometric methylation assay, LUMA

K Luttropp, LK Sjöholm, TJ Ekström - Pyrosequencing: Methods and …, 2015 - Springer
The study of epigenetic alterations of the genome is becoming increasingly important in
order to understand how environment and genetic background interact to build and regulate …

Methylation-free site patterns along a 1-Mb locus on Chr19 in cancerous and normal cells are similar. A new fast approach for analyzing unmethylated CCGG sites …

T Azhikina, I Gainetdinov, Y Skvortsova… - Molecular Genetics and …, 2006 - Springer
We describe a newly developed technique for rapid identification of positions of genomic
DNA breaks, preexisting or introduced by specific digestion, in particular, by restriction …

Dam methylase accessibility as an instrument for analysis of mammalian chromatin structure

SS Bulanenkova, AA Kozlova, ES Kotova… - Epigenetics, 2011 - Taylor & Francis
For a 140-kb human genome locus, an analysis of the distribution of Dam methylase
accessible sites, DNase I sensitive and resistant regions, unmethylated CpG sites and …