[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts

C Bernal, K Rodriguez, R Martinez - Biotechnology Advances, 2018 - Elsevier
Enzyme immobilization often achieves reusable biocatalysts with improved operational
stability and solvent resistance. However, these modifications are generally associated with …

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres… - Biotechnology …, 2015 - Elsevier
In this review, we detail the efforts performed to couple the purification and the
immobilization of industrial enzymes in a single step. The use of antibodies, the …

Importance of the support properties for immobilization or purification of enzymes

JCS Santos, O Barbosa, C Ortiz… - …, 2015 - Wiley Online Library
Immobilization and purification of enzymes are usual requirements for their industrial use.
Both purification and immobilization have a common factor: they use a solid activated …

Potential of different enzyme immobilization strategies to improve enzyme performance

C Garcia‐Galan, Á Berenguer‐Murcia… - Advanced Synthesis …, 2011 - Wiley Online Library
Enzyme biocatalysis plays a very relevant role in the development of many chemical
industries, eg, energy, food or fine chemistry. To achieve this goal, enzyme immobilization is …

Inorganic materials as supports for covalent enzyme immobilization: methods and mechanisms

P Zucca, E Sanjust - Molecules, 2014 - mdpi.com
Several inorganic materials are potentially suitable for enzymatic covalent immobilization, by
means of several different techniques. Such materials must meet stringent criteria to be …

Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties

O Barbosa, R Torres, C Ortiz… - …, 2013 - ACS Publications
A heterofunctional support for enzyme immobilization may be defined as that which
possesses several distinct functionalities on its surface able to interact with a protein. We will …

Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance

K Hernandez, R Fernandez-Lafuente - Enzyme and microbial technology, 2011 - Elsevier
Mutagenesis and immobilization are usually considered to be unrelated techniques with
potential applications to improve protein properties. However, there are several reports …

“Smart” chemistry and its application in peroxidase immobilization using different support materials

M Bilal, T Rasheed, Y Zhao, HMN Iqbal, J Cui - International journal of …, 2018 - Elsevier
In the past few decades, the enzyme immobilization technology has been exploited a lot and
thus became a matter of rational design. Immobilization is an alternative approach to bio …

Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization

DA Cowan, R Fernandez-Lafuente - Enzyme and Microbial Technology, 2011 - Elsevier
The immobilization of proteins (mostly typically enzymes) onto solid supports is mature
technology and has been used successfully to enhance biocatalytic processes in a wide …