Phage proteins target and co-opt host ribosomes immediately upon infection

M Gerovac, K Chihara, L Wicke, B Böttcher… - Nature …, 2024 - nature.com
Bacteriophages must seize control of the host gene expression machinery to replicate. To
bypass bacterial anti-phage defence systems, this host takeover occurs immediately upon …

The intricate organizational strategy of nucleus-forming phages

A Prichard, J Pogliano - Current Opinion in Microbiology, 2024 - Elsevier
Nucleus-forming phages (chimalliviruses) encode numerous genes responsible for creating
intricate structures for viral replication. Research on this newly appreciated family of phages …

Nucleus-forming vibriophage cocktail reduces shrimp mortality in the presence of pathogenic bacteria

K Thammatinna, A Sinprasertporn, A Naknaen… - Scientific Reports, 2023 - nature.com
The global aquaculture industry has suffered significant losses due to the outbreak of Acute
Hepatopancreatic Necrosis Disease (AHPND) caused by Vibrio parahaemolyticus. Since …

A phage nucleus-associated RNA-binding protein is required for jumbo phage infection

E Enustun, EG Armbruster, J Lee, S Zhang… - Nucleic Acids …, 2024 - academic.oup.com
Large-genome bacteriophages (jumbo phages) of the proposed family Chimalliviridae
assemble a nucleus-like compartment bounded by a protein shell that protects the …

An intron endonuclease facilitates interference competition between coinfecting viruses

EA Birkholz, CJ Morgan, TG Laughlin, RK Lau… - Science, 2024 - science.org
Introns containing homing endonucleases are widespread in nature and have long been
assumed to be selfish elements that provide no benefit to the host organism. These genetic …

[HTML][HTML] Sequential membrane-and protein-bound organelles compartmentalize genomes during phage infection

EG Armbruster, J Lee, J Hutchings, AR VanderWal… - bioRxiv, 2023 - ncbi.nlm.nih.gov
Eukaryotic viruses assemble compartments required for genome replication, but no such
organelles are known to be essential for prokaryotic viruses. Bacteriophages of the family …

Genomic transfer via membrane vesicle: A strategy of giant phage phiKZ for early infection

D Antonova, A Nichiporenko, M Sobinina… - Journal of …, 2024 - journals.asm.org
During infection, the giant phiKZ phage forms a specialized structure at the center of the host
cell called the phage nucleus. This structure is crucial for safeguarding viral DNA against …

An essential and highly selective protein import pathway encoded by nucleus-forming phage

CJ Morgan, E Enustun, EG Armbruster… - Proceedings of the …, 2024 - National Acad Sciences
Targeting proteins to specific subcellular destinations is essential in prokaryotes,
eukaryotes, and the viruses that infect them. Chimalliviridae phages encapsulate their …

Jumbo phages are active against extensively drug-resistant eyedrop-associated Pseudomonas aeruginosa infections

AG Cobián Güemes, P Ghatbale… - Antimicrobial Agents …, 2023 - Am Soc Microbiol
Antibiotic-resistant bacteria present an emerging challenge to human health. Their
prevalence has been increasing across the globe due in part to the liberal use of antibiotics …

Genome-wide characterization of diverse bacteriophages enabled by RNA-binding CRISPRi

BA Adler, MJ Al-Shimary, JR Patel, E Armbruster… - bioRxiv, 2023 - biorxiv.org
Bacteriophages constitute one of the largest sources of unknown gene content in the
biosphere. Even for well-studied model phages, robust experimental approaches to identify …