Highlights•Hybrid-FRET on rails automates FRET-based integrative structural
modeling.•Automated experiment design assures highest structural quality with minimal …

Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …

Single-molecule Förster-resonance energy transfer (smFRET) experiments allow the study
of biomolecular structure and dynamics in vitro and in vivo. We performed an international …

Experimental, theoretical, and computational studies of small proteins suggest that
interresidue contacts not present in the folded structure play little or no role in the self …

Single-molecule fluorescence microscopy is uniquely suited for detecting transient
molecular recognition events, yet achieving the time resolution and statistics needed to …

Highlights•Single molecule FRET experiments determine average transition path
times.•Observed times are extremely close to those found in all-atom MD simulations.•Times …

Monomers of amyloid-β (Aβ) protein are known to be disordered, but there is considerable
controversy over the existence of residual or transient conformations that can potentially …

Conformational dynamics of biomolecules are of fundamental importance for their function.
Single-molecule studies of Förster Resonance Energy Transfer (smFRET) between a …

Super-resolution microscopy provides direct insight into fundamental biological processes
occurring at length scales smaller than light's diffraction limit. The analysis of data at such …

Modern experimental kinetics of protein folding began in the early 1990s with the
introduction of nanosecond laser pulses to trigger the folding reaction, providing an almost …