Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical
technique that can be used to determine the structure of biological macromolecules and …

Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …

Single-particle cryogenic electron microscopy (cryo-EM) allows reconstruction of high-
resolution structures of proteins in different conformations. Protein function often involves …

All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study
short-lived molecular states. Using piezoelectric dispensing, two independent streams of …

Cryo‐electron microscopy (cryo‐EM) is a structural biological method that is used to
determine the 3D structures of biomacromolecules. After years of development, cryo‐EM has …

Cryogenic electron microscopy (cryo-EM) was the basis for the 2017 Nobel Prize in
Chemistry for its profound impact on the field of structural biology by freezing and stabilizing …

The initiation of bacterial translation involves the tightly regulated joining of the 50S
ribosomal subunit to an initiator transfer RNA (fMet-tRNAfMet)-containing 30S ribosomal …

Mechanistic understanding of biochemical reactions requires structural and kinetic
characterization of the underlying chemical processes. However, no single experimental …