Overlap** genes in natural and engineered genomes

BW Wright, MP Molloy, PR Jaschke - Nature Reviews Genetics, 2022 - nature.com
Modern genome-scale methods that identify new genes, such as proteogenomics and
ribosome profiling, have revealed, to the surprise of many, that overlap in genes, open …

Stability, robustness, and containment: preparing synthetic biology for real-world deployment

S Kumar, J Hasty - Current Opinion in Biotechnology, 2023 - Elsevier
Highlights•Environmentally responsive survival circuitry enable synthetic cell containment.•
Increased genetic stability by engineering sequence and host context.•Control circuits for …

Fighting the battle against evolution: designing genetically modified organisms for evolutionary stability

M Arbel-Groissman, I Menuhin-Gruman, D Naki… - Trends in …, 2023 - cell.com
Synthetic biology has made significant progress in many areas, but a major challenge that
has received limited attention is the evolutionary stability of synthetic constructs made of …

The design and engineering of synthetic genomes

JS James, J Dai, WL Chew, Y Cai - Nature Reviews Genetics, 2024 - nature.com
Synthetic genomics seeks to design and construct entire genomes to mechanistically dissect
fundamental questions of genome function and to engineer organisms for diverse …

Prolonging genetic circuit stability through adaptive evolution of overlap** genes

JL Chlebek, SP Leonard, C Kang-Yun… - Nucleic Acids …, 2023 - academic.oup.com
The development of synthetic biological circuits that maintain functionality over application-
relevant time scales remains a significant challenge. Here, we employed synthetic …

Intestinal engineered probiotics as living therapeutics: chassis selection, colonization enhancement, gene circuit design, and biocontainment

Y Huang, X Lin, S Yu, R Chen, W Chen - ACS synthetic biology, 2022 - ACS Publications
Intestinal probiotics are often used for the in situ treatment of diseases, such as metabolic
disorders, tumors, and chronic inflammatory infections. Recently, there has been an …

Metabolic engineering for large‐scale environmental bioremediation

PI Nikel, V de Lorenzo - Metabolic Engineering: Concepts and …, 2021 - Wiley Online Library
Designing biological agents for counteracting the noxious effects of chemical pollutants
includes–but goes beyond–engineering biodegradative pathways in suitable microbial …

GENTANGLE: integrated computational design of gene entanglements

JM Martí, C Hsu, C Rochereau, C Xu… - …, 2024 - academic.oup.com
The design of two overlap** genes in a microbial genome is an emerging technique for
adding more reliable control mechanisms in engineered organisms for increased stability …

GENTANGLE: integrated computational design of gene entanglements

JM Martí, C Hsu, C Rochereau, C Xu… - …, 2024 - academic.oup.com
The design of two overlap** genes in a microbial genome is an emerging technique for
adding more reliable control mechanisms in engineered organisms for increased stability …

Creating overlap** genes by alternate-frame insertion

SP Leonard, TM Halvorsen, B Lim, D Park, Y Jiao… - bioRxiv, 2024 - biorxiv.org
Overlap** genes, wherein two different proteins are translated from alternative frames of
the same DNA sequence, provide a means to stabilize an engineered gene by directly …