Imaging cell biology in live animals: ready for prime time

R Weigert, N Porat-Shliom… - Journal of Cell Biology, 2013 - rupress.org
Time-lapse fluorescence microscopy is one of the main tools used to image subcellular
structures in living cells. Yet for decades it has been applied primarily to in vitro model …

Intravital microscopy: a practical guide on imaging intracellular structures in live animals

A Masedunskas, O Milberg, N Porat-Shliom… - …, 2012 - Taylor & Francis
Intravital microscopy is an extremely powerful tool that enables imaging several biological
processes in live animals. Recently, the ability to image subcellular structures in several …

Intravital third harmonic generation microscopy of collective melanoma cell invasion: Principles of interface guidance and microvesicle dynamics

B Weigelin, GJ Bakker, P Friedl - IntraVital, 2012 - Taylor & Francis
Cancer cell invasion is an adaptive process based on cell-intrinsic properties to migrate
individually or collectively, and their adaptation to encountered tissue structure acting as …

Label-free optical imaging in developmental biology

S Wang, IV Larina, KV Larin - Biomedical Optics Express, 2020 - opg.optica.org
Application of optical imaging in developmental biology marks an exciting frontier in
biomedical optics. Optical resolution and imaging depth allow for investigation of growing …

Multiscale label-free imaging of fibrillar collagen in the tumor microenvironment

MS Nelson, Y Liu, HM Wilson, B Li… - … : Methods and Protocols, 2023 - Springer
With recent advances in cancer therapeutics, there is a great need for improved imaging
methods for characterizing cancer onset and progression in a quantitative and actionable …

[HTML][HTML] From zygote to blastocyst: application of ultrashort lasers in the field of assisted reproduction and developmental biology

I Ilina, D Sitnikov - Diagnostics, 2021 - mdpi.com
Although the use of lasers in medical diagnosis and therapies, as well as in fundamental
biomedical research is now almost routine, advanced laser sources and new laser-based …

Nonlinear imaging techniques as non-destructive, high-resolution diagnostic tools for cultural heritage studies

G Filippidis, GJ Tserevelakis, A Selimis, C Fotakis - Applied Physics A, 2015 - Springer
Here, we present a review of the implementation of nonlinear imaging microscopy
techniques such as second and third harmonic generation (SHG-THG) and multi-photon …

Third Harmonic Generation microscopy as a reliable diagnostic tool for evaluating lipid body modification during cell activation: The example of BV-2 microglia cells

E Gavgiotaki, G Filippidis, M Kalognomou… - Journal of structural …, 2015 - Elsevier
Nonlinear optical processes have found widespread applications in fields ranging from
fundamental physics to biomedicine. In this study, we attempted to evaluate cell activation by …

Multi photon excitation fluorescence imaging microscopy for the precise characterization of corrosion layers in silver-based artifacts

F Faraldi, GJ Tserevelakis, G Filippidis, GM Ingo… - Applied Physics A, 2013 - Springer
In this study, we present results of the implementation of multi photon excitation fluorescence
(MPEF) imaging measurements to silver-based artifacts for the identification of the corrosion …

Femtosecond laser nanosurgery of sub‐cellular structures in HeLa cells by employing Third Harmonic Generation imaging modality as diagnostic tool

GJ Tserevelakis, S Psycharakis, B Resan… - Journal of …, 2012 - Wiley Online Library
Femtosecond laser assisted nanosurgery of microscopic biological specimens is a relatively
new technique which allows the selective disruption of sub‐cellular structures without …