Cross-linking mass spectrometry for investigating protein conformations and protein–protein interactions─ a method for all seasons

L Piersimoni, PL Kastritis, C Arlt, A Sinz - Chemical Reviews, 2021 - ACS Publications
Mass spectrometry (MS) has become one of the key technologies of structural biology. In this
review, the contributions of chemical cross-linking combined with mass spectrometry (XL …

Cross-linking mass spectrometry: methods and applications in structural, molecular and systems biology

FJ O'Reilly, J Rappsilber - Nature structural & molecular biology, 2018 - nature.com
Over the past decade, cross-linking mass spectrometry (CLMS) has developed into a robust
and flexible tool that provides medium-resolution structural information. CLMS data provide …

A high-speed search engine pLink 2 with systematic evaluation for proteome-scale identification of cross-linked peptides

ZL Chen, JM Meng, Y Cao, JL Yin, RQ Fang… - Nature …, 2019 - nature.com
We describe pLink 2, a search engine with higher speed and reliability for proteome-scale
identification of cross-linked peptides. With a two-stage open search strategy facilitated by …

Cross-linking mass spectrometry (XL-MS): An emerging technology for interactomics and structural biology

C Yu, L Huang - Analytical chemistry, 2017 - pmc.ncbi.nlm.nih.gov
Protein-protein interactions are fundamental to the formation of intricate interaction networks
and the assembly of multisubunit protein complexes that represent the functional …

Molecular damage in aging

VN Gladyshev, SB Kritchevsky, SG Clarke, AM Cuervo… - Nature Aging, 2021 - nature.com
Cellular metabolism and environmental interactions generate molecular damage affecting
all levels of biological organization. Accumulation of this damage over time is thought to …

Trans-proteomic pipeline: robust mass spectrometry-based proteomics data analysis suite

EW Deutsch, L Mendoza, DD Shteynberg… - Journal of proteome …, 2023 - ACS Publications
The Trans-Proteomic Pipeline (TPP) mass spectrometry data analysis suite has been in
continual development and refinement since its first tools, PeptideProphet and …

A cross-linking/mass spectrometry workflow based on MS-cleavable cross-linkers and the MeroX software for studying protein structures and protein–protein …

C Iacobucci, M Götze, CH Ihling, C Piotrowski, C Arlt… - Nature protocols, 2018 - nature.com
Chemical cross-linking in combination with mass spectrometric analysis of the created cross-
linked products is an emerging technology aimed at deriving valuable structural information …

Leveraging crosslinking mass spectrometry in structural and cell biology

A Graziadei, J Rappsilber - Structure, 2022 - cell.com
Crosslinking mass spectrometry (crosslinking-MS) is a versatile tool providing structural
insights into protein conformation and protein-protein interactions. Its medium-resolution …

Mitochondrial protein interactome elucidated by chemical cross-linking mass spectrometry

DK Schweppe, JD Chavez, CF Lee… - Proceedings of the …, 2017 - National Acad Sciences
Mitochondrial protein interactions and complexes facilitate mitochondrial function. These
complexes range from simple dimers to the respirasome supercomplex consisting of …

Computational methods for understanding mass spectrometry–based shotgun proteomics data

P Sinitcyn, JD Rudolph, J Cox - Annual Review of Biomedical …, 2018 - annualreviews.org
Computational proteomics is the data science concerned with the identification and
quantification of proteins from high-throughput data and the biological interpretation of their …