An automated cell-free workflow for transcription factor engineering

HM Ekas, B Wang, AD Silverman, JB Lucks… - ACS synthetic …, 2024 - ACS Publications
The design and optimization of metabolic pathways, genetic systems, and engineered
proteins rely on high-throughput assays to streamline design-build-test-learn cycles …

Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors

F Masotti, N Krink, N Lencina, N Gottig… - ACS Synthetic …, 2024 - ACS Publications
Phosphonates (PHTs), organic compounds with a stable C–P bond, are widely distributed in
nature. Glyphosate (GP), a synthetic PHT, is extensively used in agriculture and has been …

[HTML][HTML] In Vitro Removal of Cd and Pb Through the Bacterial Strain Burkholderia zhejiangensis CEIB S4-3: Efficiency and Cellular Mechanisms Implicated in the …

AA Hernández-Guerrero, ML Castrejón-Godínez… - Processes, 2025 - mdpi.com
Heavy metals are among the most toxic environmental pollutants. They are a group of
chemical elements broadly distributed in different environments. Anthropogenic activities …

Scalable cell-free production of active T7 RNA polymerase

RN Rezvani, R Aw, W Chan, K Satish, H Chen, A Lavy… - bioRxiv, 2024 - biorxiv.org
The SARS-CoV-2 pandemic highlighted the urgent need for biomanufacturing paradigms
that are robust and fast. Here, we demonstrate the rapid process development and scalable …

Development of cell-free platforms for discovering, characterizing, and engineering post-translational modifications

DA Wong, ZM Shaver, MD Cabezas, M Daniel-Ivad… - bioRxiv, 2024 - biorxiv.org
Post-translational modifications (PTMs) are important for the stability and function of many
therapeutic proteins and peptides. Current methods for studying and engineering PTM …