Reference genes in real-time PCR
B Kozera, M Rapacz - Journal of applied genetics, 2013 - Springer
This paper aims to discuss various aspects of the use of reference genes in qPCR technique
used in the thousands of present studies. Most frequently, these are housekee** genes …
used in the thousands of present studies. Most frequently, these are housekee** genes …
Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references
S Guenin, M Mauriat, J Pelloux… - Journal of …, 2009 - academic.oup.com
Quantitative RT-PCR (reverse transcription polymerase chain reaction, also known as qRT-
PCR or real-time RT-PCR) has been used in large proportions of transcriptome analyses …
PCR or real-time RT-PCR) has been used in large proportions of transcriptome analyses …
Quantification of mRNA using real-time RT-PCR
T Nolan, RE Hands, SA Bustin - Nature protocols, 2006 - nature.com
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the
evident requirement for quantitative data analysis in molecular medicine, biotechnology …
evident requirement for quantitative data analysis in molecular medicine, biotechnology …
Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process
Background The elucidation of gene expression patterns leads to a better understanding of
biological processes. Real-time quantitative RT-PCR has become the standard method for …
biological processes. Real-time quantitative RT-PCR has become the standard method for …
An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development
Background Accuracy in quantitative real-time RT-PCR is dependent on high quality RNA,
consistent cDNA synthesis, and validated stable reference genes for data normalization …
consistent cDNA synthesis, and validated stable reference genes for data normalization …
Selection of reliable reference genes for gene expression studies in peach using real-time PCR
Z Tong, Z Gao, F Wang, J Zhou, Z Zhang - BMC molecular biology, 2009 - Springer
Background RT-qPCR is a preferred method for rapid and reliable quantification of gene
expression studies. Appropriate application of RT-qPCR in such studies requires the use of …
expression studies. Appropriate application of RT-qPCR in such studies requires the use of …
Characterization of housekee** genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment
AT McCurley, GV Callard - BMC molecular biology, 2008 - Springer
Background Research using the zebrafish model has experienced a rapid growth in recent
years. Although real-time reverse transcription PCR (QPCR), normalized to an internal …
years. Although real-time reverse transcription PCR (QPCR), normalized to an internal …
Super-resolution microscopy reveals ultra-low CD19 expression on myeloma cells that triggers elimination by CD19 CAR-T
Immunotherapy with chimeric antigen receptor-engineered T-cells (CAR-T) is under
investigation in multiple myeloma. There are reports of myeloma remission after CD19 CAR …
investigation in multiple myeloma. There are reports of myeloma remission after CD19 CAR …
Altered expression of glutamate signaling, growth factor, and glia genes in the locus coeruleus of patients with major depression
Several studies have proposed that brain glutamate signaling abnormalities and glial
pathology have a role in the etiology of major depressive disorder (MDD). These …
pathology have a role in the etiology of major depressive disorder (MDD). These …
Selection and validation of a set of reliable reference genes for quantitative sod gene expression analysis in C. elegans
D Hoogewijs, K Houthoofd, F Matthijssens… - BMC molecular …, 2008 - Springer
Abstract Background In the nematode Caenorhabditis elegans the conserved Ins/IGF-1
signaling pathway regulates many biological processes including life span, stress response …
signaling pathway regulates many biological processes including life span, stress response …