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Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and cellular processes
Green fluorescent protein (GFP) from jellyfish Aequorea victoria is the most extensively
studied and widely used in cell biology protein. GFP-like proteins constitute a fast growing …
studied and widely used in cell biology protein. GFP-like proteins constitute a fast growing …
Beta-barrel scaffold of fluorescent proteins: folding, stability and role in chromophore formation
OV Stepanenko, OV Stepanenko, IM Kuznetsova… - International review of …, 2013 - Elsevier
This review focuses on the current view of the interaction between the β-barrel scaffold of
fluorescent proteins and their unique chromophore located in the internal helix. The …
fluorescent proteins and their unique chromophore located in the internal helix. The …
Chemically stable fluorescent proteins for advanced microscopy
We report the rational engineering of a remarkably stable yellow fluorescent protein
(YFP),'hyperfolder YFP'(hfYFP), that withstands chaotropic conditions that denature most …
(YFP),'hyperfolder YFP'(hfYFP), that withstands chaotropic conditions that denature most …
Engineering and characterization of a superfolder green fluorescent protein
Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions
with other proteins. We have generated a robustly folded version of GFP …
with other proteins. We have generated a robustly folded version of GFP …
Selective magnetic nanoheating: Combining iron oxide nanoparticles for multi-hot-spot induction and sequential regulation
The contactless heating capacity of magnetic nanoparticles (MNPs) has been exploited in
fields such as hyperthermia cancer therapy, catalysis, and enzymatic thermal regulation …
fields such as hyperthermia cancer therapy, catalysis, and enzymatic thermal regulation …
GFP-like proteins stably accumulate in lysosomes
H Katayama, A Yamamoto, N Mizushima… - Cell structure and …, 2008 - jstage.jst.go.jp
GFPs), and more recently the novel GFP-like proteins from Anthozoa have greatly advanced
our technologies for fluorescently labeling cells, organelles, and proteins. It has been …
our technologies for fluorescently labeling cells, organelles, and proteins. It has been …
Crystal structure of enhanced green fluorescent protein to 1.35 Å resolution reveals alternative conformations for Glu222
JAJ Arpino, PJ Rizkallah, DD Jones - 2012 - journals.plos.org
Enhanced Green Fluorescent Protein (EGFP) is one of the most widely used engineered
variants of the original wild-type Green Fluorescent Protein. Here, we report the high …
variants of the original wild-type Green Fluorescent Protein. Here, we report the high …
Fluorescent protein lifetimes report densities and phases of nuclear condensates during embryonic stem-cell differentiation
Fluorescent proteins (FP) are frequently used for studying proteins inside cells. In advanced
fluorescence microscopy, FPs can report on additional intracellular variables. One variable …
fluorescence microscopy, FPs can report on additional intracellular variables. One variable …
Real-time imaging of discrete exocytic events mediating surface delivery of AMPA receptors
GA Yudowski, MA Puthenveedu… - Journal of …, 2007 - jneurosci.org
We directly resolved discrete exocytic fusion events mediating insertion of AMPA-type
glutamate receptors (AMPARs) to the somatodendritic surface of rat hippocampal pyramidal …
glutamate receptors (AMPARs) to the somatodendritic surface of rat hippocampal pyramidal …
Swee** away protein aggregation with entropic bristles: intrinsically disordered protein fusions enhance soluble expression
AA Santner, CH Croy, FH Vasanwala, VN Uversky… - Biochemistry, 2012 - ACS Publications
Intrinsically disordered, highly charged protein sequences act as entropic bristles (EBs),
which, when translationally fused to partner proteins, serve as effective solubilizers by …
which, when translationally fused to partner proteins, serve as effective solubilizers by …