The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …

In bacteria and archaea, clustered regularly interspaced short palindromic repeats
(CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system …

We recently developed base editing, the programmable conversion of target C: G base pairs
to T: A without inducing double-stranded DNA breaks (DSBs) or requiring homology …

Class 2 CRISPR–Cas systems are characterized by effector modules that consist of a single
multidomain protein, such as Cas9 or Cpf1. We designed a computational pipeline for the …

Current genome-editing technologies introduce double-stranded (ds) DNA breaks at a target
locus as the first step to gene correction,. Although most genetic diseases arise from point …

CRISPR–Cas systems that provide defence against mobile genetic elements in bacteria and
archaea have evolved a variety of mechanisms to target and cleave RNA or DNA. The well …

The CRISPR-associated protein Cas12a (Cpf1), which has been repurposed for genome
editing, possesses two distinct nuclease activities: endoribonuclease activity for processing …

Bacteria and archaea possess a range of defense mechanisms to combat plasmids and viral
infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short …

BACKGROUND Prokaryotes have evolved multiple systems to combat invaders such as
viruses and plasmids. Examples of such defense systems include receptor masking …

Although polymerase chain reaction (PCR) is the most widely used method for DNA
amplification, the requirement of thermocycling limits its non-laboratory applications …