Applications of single-cell RNA sequencing in drug discovery and development

B Van de Sande, JS Lee, E Mutasa-Gottgens… - Nature Reviews Drug …, 2023 - nature.com
Single-cell technologies, particularly single-cell RNA sequencing (scRNA-seq) methods,
together with associated computational tools and the growing availability of public data …

[HTML][HTML] The human tumor atlas network: charting tumor transitions across space and time at single-cell resolution

O Rozenblatt-Rosen, A Regev, P Oberdoerffer, T Nawy… - Cell, 2020 - cell.com
Crucial transitions in cancer—including tumor initiation, local expansion, metastasis, and
therapeutic resistance—involve complex interactions between cells within the dynamic …

COVID-19 tissue atlases reveal SARS-CoV-2 pathology and cellular targets

TM Delorey, CGK Ziegler, G Heimberg, R Normand… - Nature, 2021 - nature.com
COVID-19, which is caused by SARS-CoV-2, can result in acute respiratory distress
syndrome and multiple organ failure,,–, but little is known about its pathophysiology. Here …

Spatially organized multicellular immune hubs in human colorectal cancer

K Pelka, M Hofree, JH Chen, S Sarkizova, JD Pirl… - Cell, 2021 - cell.com
Immune responses to cancer are highly variable, with mismatch repair-deficient (MMRd)
tumors exhibiting more anti-tumor immunity than mismatch repair-proficient (MMRp) tumors …

Single-nucleus cross-tissue molecular reference maps toward understanding disease gene function

G Eraslan, E Drokhlyansky, S Anand, E Fiskin… - Science, 2022 - science.org
Understanding gene function and regulation in homeostasis and disease requires
knowledge of the cellular and tissue contexts in which genes are expressed. Here, we …

Tissue-resident memory and circulating T cells are early responders to pre-surgical cancer immunotherapy

AM Luoma, S Suo, Y Wang, L Gunasti, CBM Porter… - Cell, 2022 - cell.com
Neoadjuvant immune checkpoint blockade has shown promising clinical activity. Here, we
characterized early kinetics in tumor-infiltrating and circulating immune cells in oral cancer …

Joint probabilistic modeling of single-cell multi-omic data with totalVI

A Gayoso, Z Steier, R Lopez, J Regier, KL Nazor… - Nature …, 2021 - nature.com
The paired measurement of RNA and surface proteins in single cells with cellular indexing
of transcriptomes and epitopes by sequencing (CITE-seq) is a promising approach to …

Single-cell biological network inference using a heterogeneous graph transformer

A Ma, X Wang, J Li, C Wang, T **ao, Y Liu… - Nature …, 2023 - nature.com
Single-cell multi-omics (scMulti-omics) allows the quantification of multiple modalities
simultaneously to capture the intricacy of complex molecular mechanisms and cellular …

The landscape of immune dysregulation in Crohn's disease revealed through single-cell transcriptomic profiling in the ileum and colon

L Kong, V Pokatayev, A Lefkovith, GT Carter… - Immunity, 2023 - cell.com
Crohn's disease (CD) is a chronic gastrointestinal disease that is increasing in prevalence
worldwide. CD is multifactorial, involving the complex interplay of genetic, immune, and …