The biotechnological applications of enzymes are limited due to the activity–stability trade-
off, which implies that an increase in activity is accompanied by a concomitant decrease in …

Enzymes from psychrophilic (cold-loving) organisms have attracted considerable interest
over the past decades for their potential in various low-temperature industrial processes …

Glucose oxidase (Gox) is a biocatalyst that is widely applied in the food industry, as well as
other biotechnological industries. However, the industrial application of Gox is hampered by …

The restricted availability of UDP-glucose, an essential precursor that targets
oligo/polysaccharide and glycoside synthesis, makes its practical application difficult …

The immobilized enzymes' properties can be affected by cross-linkers on the surface of
supports. To study how cross-linkers alter enzymes function, chitosan-coated magnetic …

The effect of glycerol and sorbitol on the stability of porcine pancreas trypsin was
investigated in this work. Molecular dynamics simulation and thermostability results showed …

Psychrotrophs or psychrophiles are extremophilic microorganisms that have the maximum
temperature above 20° C for growth. Since around 70% of the earth's biosphere temperature …

Extracellular neutral proteases (NPs) in Aspergillus oryzae (A. oryzae) play a role in
hydrolyzing soybean proteins into smaller peptides at pH about 7.5. The optimum pH of …

In this study, we report a detailed experimental, binding free energy calculation and
molecular dynamics (MD) simulation investigation of the interactions of carboxylic …

In this work, SVP2 from Salinivibrio proteolyticus strain AF-2004, a zinc metalloprotease with
suitable biotechnological applications, was cloned for expression at high levels in …