Functionalizing DNA origami to investigate and interact with biological systems

GA Knappe, EC Wamhoff, M Bathe - Nature Reviews Materials, 2023 - nature.com
DNA origami has emerged as a powerful method to generate DNA nanostructures with
dynamic properties and nanoscale control. These nanostructures enable complex …

In vivo applications of bioorthogonal reactions: chemistry and targeting mechanisms

MMA Mitry, F Greco, HMI Osborn - Chemistry–A European …, 2023 - Wiley Online Library
Bioorthogonal chemistry involves selective biocompatible reactions between functional
groups that are not normally present in biology. It has been used to probe biomolecules in …

Single-cell analysis and sorting using droplet-based microfluidics

L Mazutis, J Gilbert, WL Ung, DA Weitz, AD Griffiths… - Nature protocols, 2013 - nature.com
We present a droplet-based microfluidics protocol for high-throughput analysis and sorting
of single cells. Compartmentalization of single cells in droplets enables the analysis of …

Droplet microfluidics—A tool for single‐cell analysis

HN Joensson… - Angewandte Chemie …, 2012 - Wiley Online Library
Droplet microfluidics allows the isolation of single cells and reagents in monodisperse
picoliter liquid capsules and manipulations at a throughput of thousands of droplets per …

Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology

AB Theberge, F Courtois, Y Schaerli… - Angewandte Chemie …, 2010 - Wiley Online Library
Microdroplets in microfluidics offer a great number of opportunities in chemical and
biological research. They provide a compartment in which species or reactions can be …

Analytical detection techniques for droplet microfluidics—A review

Y Zhu, Q Fang - Analytica chimica acta, 2013 - Elsevier
In the last decade, droplet-based microfluidics has undergone rapid progress in the fields of
single-cell analysis, digital PCR, protein crystallization and high throughput screening. It has …

A review of the theory, methods and recent applications of high-throughput single-cell droplet microfluidics

TP Lagus, JF Edd - Journal of Physics D: Applied Physics, 2013 - iopscience.iop.org
Most cell biology experiments are performed in bulk cell suspensions where cell secretions
become diluted and mixed in a contiguous sample. Confinement of single cells to small …

Quantification of protein interactions and solution transport using high-density GMR sensor arrays

RS Gaster, L Xu, SJ Han, RJ Wilson, DA Hall… - Nature …, 2011 - nature.com
Monitoring the kinetics of protein interactions on a high-density sensor array is vital to drug
development and proteomic analysis. Label-free kinetic assays based on surface plasmon …

A CRISPR-Cas12a-powered magnetic relaxation switching biosensor for the sensitive detection of Salmonella

Y Shen, F Jia, Y He, Y Fu, W Fang, J Wang… - Biosensors and …, 2022 - Elsevier
Magnetic relaxation switching (MRS) biosensors are attractive in the field of food safety
owing to their simplicity and high signal-to-noise ratio. But they are less in sensitivity and …

Length-independent DNA packing into nanopore zero-mode waveguides for low-input DNA sequencing

J Larkin, RY Henley, V Jadhav, J Korlach… - Nature …, 2017 - nature.com
Compared with conventional methods, single-molecule real-time (SMRT) DNA sequencing
exhibits longer read lengths than conventional methods, less GC bias, and the ability to read …