Structures and functions of short argonautes

C Wang, Z Shen, XY Yang, TM Fu - RNA biology, 2024 - Taylor & Francis
Argonaute proteins (Agos) represent a highly conserved family of proteins prevalent in all
domains of life and have been implicated in various biological processes. Based on the …

Auto-inhibition and activation of a short Argonaute-associated TIR-APAZ defense system

L Guo, P Huang, Z Li, YC Shin, P Yan, M Lu… - Nature Chemical …, 2024 - nature.com
Short prokaryotic Ago accounts for most prokaryotic Argonaute proteins (pAgos) and is
involved in defending bacteria against invading nucleic acids. Short pAgo associated with …

Nucleic-acid-triggered NADase activation of a short prokaryotic Argonaute

X Gao, K Shang, K Zhu, L Wang, Z Mu, X Fu, X Yu… - Nature, 2024 - nature.com
Argonaute (Ago) proteins mediate RNA-or DNA-guided inhibition of nucleic acids,. Although
the mechanisms used by eukaryotic Ago proteins and long prokaryotic Ago proteins (pAgos) …

Structural characterization of TIR-domain signalosomes through a combination of structural biology approaches

A Bhatt, BP Mishra, W Gu, M Sorbello, H Xu, T Ve… - IUCrJ, 2024 - journals.iucr.org
The TIR (Toll/interleukin-1 receptor) domain represents a vital structural element shared by
proteins with roles in immunity signalling pathways across phyla (from humans and plants to …

Nucleic acid mediated activation of a short prokaryotic argonaute immune system

J Kottur, R Malik, AK Aggarwal - Nature Communications, 2024 - nature.com
A short prokaryotic Argonaute (pAgo) TIR-APAZ (SPARTA) defense system, activated by
invading DNA to unleash its TIR domain for NAD (P)+ hydrolysis, was recently identified in …

Asgard archaea defense systems and their roles in the origin of eukaryotic immunity

P Leão, ME Little, KE Appler, D Sahaya… - Nature …, 2024 - nature.com
Dozens of new antiviral systems have been recently characterized in bacteria. Some of
these systems are present in eukaryotes and appear to have originated in prokaryotes, but …

Target DNA-dependent activation mechanism of the prokaryotic immune system SPARTA

G Finocchio, B Koopal, A Potocnik… - Nucleic acids …, 2024 - academic.oup.com
In both prokaryotic and eukaryotic innate immune systems, TIR domains function as
NADases that degrade the key metabolite NAD+ or generate signaling molecules. Catalytic …

DNA-targeting short Argonautes complex with effector proteins for collateral nuclease activity and bacterial population immunity

M Prostova, A Kanevskaya, V Panteleev… - Nature …, 2024 - nature.com
Two prokaryotic defence systems, prokaryotic Argonautes (pAgos) and CRISPR–Cas, detect
and cleave invader nucleic acids using complementary guides and the nuclease activities of …

Insights into the modulation of bacterial NADase activity by phage proteins

H Yin, X Li, X Wang, C Zhang, J Gao, G Yu… - Nature …, 2024 - nature.com
Abstract The Silent Information Regulator 2 (SIR2) protein is widely implicated in antiviral
response by depleting the cellular metabolite NAD+. The defense-associated sirtuin 2 …

The missing part: the Archaeoglobus fulgidus Argonaute forms a functional heterodimer with an N-L1-L2 domain protein

E Manakova, E Golovinas, R Pocevičiūtė… - Nucleic Acids …, 2024 - academic.oup.com
Argonaute (Ago) proteins are present in all three domains of life (bacteria, archaea and
eukaryotes). They use small (15–30 nucleotides) oligonucleotide guides to bind …