The roles of reactive oxygen species and antioxidants in cryopreservation

JS Len, WSD Koh, SX Tan - Bioscience reports, 2019 - portlandpress.com
Cryopreservation has facilitated advancement of biological research by allowing the storage
of cells over prolonged periods of time. While cryopreservation at extremely low …

Osmotic stress and cryoinjury of koala sperm: an integrative study of the plasma membrane, chromatin stability and mitochondrial function

SD Johnston, N Satake, Y Zee… - …, 2012 - rep.bioscientifica.com
This study investigated whether cryopreservation-induced injury to koala spermatozoa could
be explained using an experimental model that mimics the structural and physiological …

Improved sperm cryosurvival in diluents containing amides versus glycerol in the Przewalski's horse (Equus ferus przewalskii)

BS Pukazhenthi, A Johnson, HD Guthrie, N Songsasen… - Cryobiology, 2014 - Elsevier
Two studies were conducted to understand sperm cryosensitivity in an endangered equid,
the Przewalski's horse (Equus ferus przewalski), while testing the cryoprotectant ability of …

Extension, osmotic tolerance and cryopreservation of saltwater crocodile (Crocodylus porosus) spermatozoa

SD Johnston, J Lever, R McLeod, E Qualischefski… - Aquaculture, 2014 - Elsevier
Semen collected from 10 saltwater crocodiles (Crocodylus porosus) was used to investigate
sperm in vitro manipulation and preservation. Preliminary studies revealed that phosphate …

[PDF][PDF] Evidence that single-stranded DNA breaks are a normal feature of koala sperm chromatin, while double-stranded DNA breaks are indicative of DNA damage

YP Zee, C Lopez-Fernández, F Arroyo, SD Johnston… - Reproduction, 2009 - academia.edu
REPRODUCTION Page 1 REPRODUCTION RESEARCH Evidence that single-stranded DNA
breaks are a normal feature of koala sperm chromatin, while double-stranded DNA breaks are …

Efficacy of short-term cold storage prior to cryopreservation of spermatozoa in a threatened lizard

L Campbell, J Clulow, B Howe, R Upton… - Reproduction …, 2021 - CSIRO Publishing
Assisted reproductive technologies (ARTs) have a significant role to play in reptile
conservation, yet are severely lacking. Previous attempts to cryopreserve spermatozoa in …

The effect of chilled storage and cryopreservation on the sperm DNA fragmentation dynamics of a captive population of koalas

SD Johnston, YP Zee, C López‐Fernández… - Journal of …, 2012 - Wiley Online Library
This experiment documented the incidence and variability of sperm characteristics found in
freshly collected and ex vivo—manipulated semen samples from a population of disease …

Cryopreservation of saltwater crocodile (Crocodylus porosus) spermatozoa

SD Johnston, E Qualischefski, J Cooper… - Reproduction …, 2017 - CSIRO Publishing
The aim of the present study was to develop a protocol for the successful cryopreservation of
Saltwater crocodile spermatozoa. Sperm cells were frozen above liquid nitrogen vapour in …

Emerging arguments for reproductive technologies in wildlife and their implications for assisted reproduction and conservation of threatened marsupials

LG Howell, RR Witt - Theriogenology, 2023 - Elsevier
Assisted reproductive technologies (ARTs) have significant potential to make a meaningful
contribution to the conservation of threatened wildlife. This is true of Australia's iconic, and …

Using the Koala (Phascolarctos cinereus) as a Case Study to Illustrate the Development of Artificial Breeding Technology in Marsupials: an Update

SD Johnston, WV Holt - Reproductive sciences in animal conservation, 2019 - Springer
The successful development and application of an assisted breeding program in any animal
relies primarily on a thorough understanding of the fundamental reproductive biology …