Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

JM Bolivar, JM Woodley… - Chemical Society …, 2022 - pubs.rsc.org
Enzyme immobilization has been develo** since the 1960s and although many industrial
biocatalytic processes use the technology to improve enzyme performance, still today we …

[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

Improvement of enzyme activity, stability and selectivity via immobilization techniques

C Mateo, JM Palomo, G Fernandez-Lorente… - Enzyme and microbial …, 2007 - Elsevier
In spite of their excellent catalytic properties, enzyme properties have to be usually improved
before their implementation at industrial scale (where many cycles of high yield processes …

Parameters necessary to define an immobilized enzyme preparation

J Boudrant, JM Woodley, R Fernandez-Lafuente - Process Biochemistry, 2020 - Elsevier
Biocatalytic processes continue to find increasing application in industry. Therefore enzyme
immobilization has also become of increasing importance as a means of allowing enzyme …

Advances in enzyme immobilisation

D Brady, J Jordaan - Biotechnology letters, 2009 - Springer
Improvements in current strategies for carrier-based immobilisation have been developed
using hetero-functionalised supports that enhance the binding efficacy and stability through …

Polyethylenimine: a very useful ionic polymer in the design of immobilized enzyme biocatalysts

JJ Virgen-Ortiz, JCS Dos Santos… - Journal of materials …, 2017 - pubs.rsc.org
This review discusses the possible roles of polyethylenimine (PEI) in the design of improved
immobilized biocatalysts from diverse perspectives. This includes their use to activate …

Coupling chemical modification and immobilization to improve the catalytic performance of enzymes

RC Rodrigues, Á Berenguer‐Murcia… - Advanced Synthesis …, 2011 - Wiley Online Library
Chemical modification and immobilization of enzymes have been usually considered
unrelated tools to improve biocatalyst features. However, there are many examples where a …

Glyoxyl agarose: a fully inert and hydrophilic support for immobilization and high stabilization of proteins

C Mateo, JM Palomo, M Fuentes, L Betancor… - Enzyme and Microbial …, 2006 - Elsevier
Glyoxyl agarose is constituted by quite thick agarose fibres containing a large number of
very stable aldehyde groups attached to the support by very short spacer arms. Under …

An up-to-date review on the remediation of dyes and phenolic compounds from wastewaters using enzymes immobilized on emerging and nanostructured materials …

MK Al-Sakkaf, I Basfer, M Iddrisu, SA Bahadi… - Nanomaterials, 2023 - mdpi.com
Addressing the critical issue of water pollution, this review article emphasizes the need to
remove hazardous dyes and phenolic compounds from wastewater. These pollutants pose …

Enhanced enzyme stability through site-directed covalent immobilization

JCY Wu, CH Hutchings, MJ Lindsay, CJ Werner… - Journal of …, 2015 - Elsevier
Breakthroughs in enzyme immobilization have enabled increased enzyme recovery and
reusability, leading to significant decreases in the cost of enzyme use and fueling …