This chapter is an introductory overview of the most commonly used assay methods to
estimate the number of viable cells in multi-well plates. This chapter describes assays where …

Most cancer biologists still rely on conventional two-dimensional (2D) monolayer culture
techniques to test in vitro anti-tumor drugs prior to in vivo testing. However, the vast majority …

Cancer cells propagated in three-dimensional (3D) culture systems exhibit physiologically
relevant cell–cell and cell–matrix interactions, gene expression and signaling pathway …

Microfluidics has recently emerged as a powerful tool in generation of submillimeter-sized
cell aggregates capable of performing tissue-specific functions, so-called microtissues, for …

The mesenchymal-amoeboid transition (MAT) was proposed as a mechanism for cancer
cells to adapt their migration mode to their environment. While the molecular pathways …

Physical forces have a crucial role in tumor progression and cancer treatment. The
application of principles of engineering and physical sciences to oncology has provided …

Abstract Three-dimensional (3D) in vitro models have been used in cancer research as an
intermediate model between in vitro cancer cell line cultures and in vivo tumor. Spherical …

Cell manipulation in droplets has emerged as one of the great successes of microfluidic
technologies, with the development of single-cell screening. However, the droplet format has …

Multicellular spheroids and organoids are promising in vitro 3D models in personalized
medicine and drug screening. They replicate the structural and functional characteristics of …

Significant advances in materials, microscale technology, and stem cell biology have
enabled the construction of 3D tissues and organs, which will ultimately lead to more …