This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from
an optics perspective. As such, emphasis is placed on the advantages that LS microscope …

Light-sheet fluorescence microscopy (LSFM) has been present in cell biology laboratories
for quite some time, mainly as custom-made systems, with imaging applications ranging …

Last decade's advancements in optofluidics allowed obtaining an ever increasing
integration of different functionalities in lab on chip devices to culture, analyze, and …

Single-cell analysis techniques are fundamental to study the heterogeneity of cellular
populations, which is the basis to understand several biomedical mechanisms. Light-sheet …

Microfluidics can precisely control and manipulate micro-scale fluids, and are also known as
lab-on-a-chip or micro total analysis systems. Microfluidics have huge application potential …

Volumetric, sub-micron to micron level resolution imaging is necessary to assay phenotypes
or characteristics at the sub-cellular/organelle scale. However, three-dimensional …

Optical imaging is arguably the most effective tool to visualize living cells with high
spatiotemporal resolution and in a nearly noninvasive manner. Driven by this capability …

In this study, a continuous cell-imaging system with subcellular resolution was developed by
integrating a microfluidic platform with lattice lightsheet microscopy (LLSM). To reduce …

Selective plane illumination microscopy can image biological samples at a high
spatiotemporal resolution. Complex sample preparation and system alignment normally limit …

Capturing the dynamics of live cell populations with nanoscale resolution poses a significant
challenge, primarily owing to the speed-resolution trade-off of existing microscopy …