The identification of molecular mechanisms underlying enzyme cold adaptation is a hot-
topic both for fundamental research and industrial applications. In the present contribution …

The investigation of the structural determinants of enzymatic temperature adaptation is a
crucial pre-requisite both in terms of fundamental research and industrial applications to …

Cold-active enzymes are produced by organisms, known as psychrophiles, adapted to
permanently cold habitats. Low temperatures have an exponential deleterious effct on …

We demonstrate the inhibition of the native phosphatase activity of a cold active alkaline
phosphatase from Vibrio (VAP)(IC50 of 44±4 (n= 4) μM at pH 7.0 after a 30min …

Alkaline phosphatase is a homodimeric metallo-hydrolase where both Zn 2+ and Mg 2+ are
important for catalysis and stability. Cold-adapted alkaline phosphatase variants have high …

BACKGROUND: Protein dynamics influence protein function and stability and modulate
conformational changes. Such motions depend on the underlying networks of intramolecular …

In recent years, increased interest has been directed to the study of enzyme adaptation to
low temperatures. In particular, a peculiar folding funnel model was proposed for the free …

Cold-active enzymes are produced by organisms adapted to permanently cold habitats. Due
to the depressive effect of low temperatures on reaction rates, these enzymes have to be …

Background Para-nitrophenyl phosphate, the common substrate for alkaline phosphatase
(AP), is available as a cyclohexylamine salt. Here, we report that cyclohexylamine is a non …

Multiple comparison of the Molecular Dynamics (MD) trajectories of mutants in a cold-
adapted α-amylase (AHA) could be used to elucidate functional features required to restore …