Predicting multiple conformations via sequence clustering and AlphaFold2

HK Wayment-Steele, A Ojoawo, R Otten, JM Apitz… - Nature, 2024 - nature.com
Abstract AlphaFold2 (ref.) has revolutionized structural biology by accurately predicting
single structures of proteins. However, a protein's biological function often depends on …

Circadian oscillators: around the transcription–translation feedback loop and on to output

JM Hurley, JJ Loros, JC Dunlap - Trends in biochemical sciences, 2016 - cell.com
From cyanobacteria to mammals, organisms have evolved timing mechanisms to adapt to
environmental changes in order to optimize survival and improve fitness. To anticipate these …

Circadian rhythms in cyanobacteria

SE Cohen, SS Golden - Microbiology and Molecular Biology …, 2015 - Am Soc Microbiol
Life on earth is subject to daily and predictable fluctuations in light intensity, temperature,
and humidity created by rotation of the earth. Circadian rhythms, generated by a circadian …

AlphaFold2 fails to predict protein fold switching

D Chakravarty, LL Porter - Protein Science, 2022 - Wiley Online Library
AlphaFold2 has revolutionized protein structure prediction by leveraging sequence
information to rapidly model protein folds with atomic‐level accuracy. Nevertheless, previous …

Prediction of multiple conformational states by combining sequence clustering with AlphaFold2

HK Wayment-Steele, S Ovchinnikov, L Colwell, D Kern - BioRxiv, 2022 - biorxiv.org
Abstract AlphaFold2 (AF2) has revolutionized structural biology by accurately predicting
single structures of proteins and protein-protein complexes. However, biological function is …

Reconstitution of an intact clock reveals mechanisms of circadian timekee**

AG Chavan, JA Swan, J Heisler, C Sancar, DC Ernst… - Science, 2021 - science.org
INTRODUCTION Circadian clocks provide an internal representation of local time inside
cells and control the timing of gene expression in anticipation of sunrise and sunset. In …

CyanoGate: a modular cloning suite for engineering cyanobacteria based on the plant MoClo syntax

R Vasudevan, GAR Gale, AA Schiavon… - Plant …, 2019 - academic.oup.com
Recent advances in synthetic biology research have been underpinned by an exponential
increase in available genomic information and a proliferation of advanced DNA assembly …

Extant fold-switching proteins are widespread

LL Porter, LL Looger - … of the National Academy of Sciences, 2018 - National Acad Sciences
A central tenet of biology is that globular proteins have a unique 3D structure under
physiological conditions. Recent work has challenged this notion by demonstrating that …

Structural basis of the day-night transition in a bacterial circadian clock

R Tseng, NF Goularte, A Chavan, J Luu, SE Cohen… - Science, 2017 - science.org
Circadian clocks are ubiquitous timing systems that induce rhythms of biological activities in
synchrony with night and day. In cyanobacteria, timing is generated by a posttranslational …

Timing the day: what makes bacterial clocks tick?

CH Johnson, C Zhao, Y Xu, T Mori - Nature Reviews Microbiology, 2017 - nature.com
Chronobiological studies of prokaryotic organisms have generally lagged far behind the
study of endogenous circadian clocks in eukaryotes, in which such systems are essentially …