Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Xanthene, cyanine, oxazine and BODIPY: the four pillars of the fluorophore empire for super-resolution bioimaging

S Samanta, K Lai, F Wu, Y Liu, S Cai, X Yang… - Chemical Society …, 2023 - pubs.rsc.org
In the realm of biological research, the invention of super-resolution microscopy (SRM) has
enabled the visualization of ultrafine sub-cellular structures and their functions in live cells at …

Super-resolution microscopy demystified

L Schermelleh, A Ferrand, T Huser, C Eggeling… - Nature cell …, 2019 - nature.com
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

Small-molecule fluorogenic probes for mitochondrial nanoscale imaging

R Zhai, B Fang, Y Lai, B Peng, H Bai, X Liu… - Chemical Society …, 2023 - pubs.rsc.org
Mitochondria are inextricably linked to the development of diseases and cell metabolism
disorders. Super-resolution imaging (SRI) is crucial in enhancing our understanding of …

Seeing beyond the limit: A guide to choosing the right super-resolution microscopy technique

J Valli, A Garcia-Burgos, LM Rooney… - Journal of Biological …, 2021 - jbc.org
Super-resolution microscopy has become an increasingly popular and robust tool across the
life sciences to study minute cellular structures and processes. However, with the increasing …

Nuclear pores as versatile reference standards for quantitative superresolution microscopy

JV Thevathasan, M Kahnwald, K Cieśliński, P Hoess… - Nature …, 2019 - nature.com
Quantitative fluorescence and superresolution microscopy are often limited by insufficient
data quality or artifacts. In this context, it is essential to have biologically relevant control …

Long-wavelength photoconvertible dimeric BODIPYs for super-resolution single-molecule localization imaging in near-infrared emission

Q Gong, X Zhang, W Li, X Guo, Q Wu… - Journal of the …, 2022 - ACS Publications
Sulfoxide-bridged dimeric BODIPYs were developed as a new class of long-wavelength
photoconvertible fluorophores. Upon visible-light irradiation, a sulfoxide moiety was …

[HTML][HTML] Chromatin fibers are formed by heterogeneous groups of nucleosomes in vivo

MA Ricci, C Manzo, MF García-Parajo, M Lakadamyali… - Cell, 2015 - cell.com
Nucleosomes help structure chromosomes by compacting DNA into fibers. To gain insight
into how nucleosomes are arranged in vivo, we combined quantitative super-resolution …

Single-molecule localization microscopy in eukaryotes

M Sauer, M Heilemann - Chemical reviews, 2017 - ACS Publications
Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

Switchable fluorophores for single-molecule localization microscopy

H Li, JC Vaughan - Chemical reviews, 2018 - ACS Publications
The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …