Upcoming progress of transcriptomics studies on plants: An overview

P Tyagi, D Singh, S Mathur, A Singh… - Frontiers in Plant …, 2022 - frontiersin.org
Transcriptome sequencing or RNA-Sequencing is a high-resolution, sensitive and high-
throughput next-generation sequencing (NGS) approach used to study non-model plants …

Advances and challenges in metatranscriptomic analysis

M Shakya, CC Lo, PSG Chain - Frontiers in genetics, 2019 - frontiersin.org
Sequencing-based analyses of microbiomes have traditionally focused on addressing the
question of community membership and profiling taxonomic abundance through amplicon …

ExpressAnalyst: A unified platform for RNA-sequencing analysis in non-model species

P Liu, J Ewald, Z Pang, E Legrand, YS Jeon… - Nature …, 2023 - nature.com
The increasing application of RNA sequencing to study non-model species demands easy-
to-use and efficient bioinformatics tools to help researchers quickly uncover biological and …

Transcriptome assembly from long-read RNA-seq alignments with StringTie2

S Kovaka, AV Zimin, GM Pertea, R Razaghi… - Genome biology, 2019 - Springer
RNA sequencing using the latest single-molecule sequencing instruments produces reads
that are thousands of nucleotides long. The ability to assemble these long reads can greatly …

Accuracy assessment of fusion transcript detection via read-map** and de novo fusion transcript assembly-based methods

BJ Haas, A Dobin, B Li, N Stransky, N Pochet, A Regev - Genome biology, 2019 - Springer
Background Accurate fusion transcript detection is essential for comprehensive
characterization of cancer transcriptomes. Over the last decade, multiple bioinformatic tools …

rnaSPAdes: a de novo transcriptome assembler and its application to RNA-Seq data

E Bushmanova, D Antipov, A Lapidus… - GigaScience, 2019 - academic.oup.com
Background The possibility of generating large RNA-sequencing datasets has led to
development of various reference-based and de novo transcriptome assemblers with their …

Lipid exchanges drove the evolution of mutualism during plant terrestrialization

MK Rich, N Vigneron, C Libourel, J Keller, L Xue… - Science, 2021 - science.org
Symbiosis with arbuscular mycorrhizal fungi (AMF) improves plant nutrition in most land
plants, and its contribution to the colonization of land by plants has been hypothesized …

Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown

M Pertea, D Kim, GM Pertea, JT Leek, SL Salzberg - Nature protocols, 2016 - nature.com
High-throughput sequencing of mRNA (RNA-seq) has become the standard method for
measuring and comparing the levels of gene expression in a wide variety of species and …

A tissue-mapped axolotl de novo transcriptome enables identification of limb regeneration factors

DM Bryant, K Johnson, T DiTommaso, T Tickle… - Cell reports, 2017 - cell.com
Mammals have extremely limited regenerative capabilities; however, axolotls are profoundly
regenerative and can replace entire limbs. The mechanisms underlying limb regeneration …

[PDF][PDF] A simple guide to de novo transcriptome assembly and annotation

V Raghavan, L Kraft, F Mesny… - Briefings in …, 2022 - academic.oup.com
A transcriptome constructed from short-read RNA sequencing (RNA-seq) is an easily
attainable proxy catalog of protein-coding genes when genome assembly is unnecessary …