Water solubility and structural stability are key merits for proteins defined by the primary
sequence and 3D-conformation. Their manipulation represents important aspects of the …

Crystallography of nanocrystalline materials has witnessed a true revolution in the past 10
years, thanks to the introduction of protocols for 3D acquisition and analysis of electron …

Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical
technique that can be used to determine the structure of biological macromolecules and …

Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

Amyloid-β (Aβ) is a 39–42 residue protein produced by the cleavage of the amyloid
precursor protein (APP), which subsequently aggregates to form cross-β amyloid fibrils that …

Biological membranes are tricky to investigate. They are complex in terms of molecular
composition and structure, functional over a wide range of time scales, and characterized by …

Abstract Three-dimensional (3D) structure determination by single particle electron
cryomicroscopy (cryoEM) involves the calculation of an initial 3D model, followed by …

Since determination of the myoglobin structure in 1957, X-ray crystallography, as the
anchoring tool of structural biology, has played an instrumental role in deciphering the …

In 2013 we established a cryo-electron microscopy (cryo-EM) technique called microcrystal
electron diffraction (MicroED). Since that time, data collection and analysis schemes have …

The “Gold Standard” in Biochemistry text books. Biochemistry 4e, is a modern classic that
has been thoroughly revised. Don and Judy Voet explain biochemical concepts while …