Photochemical stability is one of the most important parameters that determine the
usefulness of organic dyes in different applications. This Review addresses key factors that …

Super-resolution fluorescence imaging by photoactivation or photoswitching of single
fluorophores and position determination (single-molecule localization microscopy, SMLM) …

DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) is a super-
resolution fluorescence microscopy technique that achieves single-molecule 'blinking'by …

Advances in super-resolution microscopy have demonstrated single-molecule localization
precisions of a few nanometers. However, translation of such high localization precisions …

The outer membrane is a formidable barrier that protects Gram-negative bacteria against
environmental threats. Its integrity requires the correct folding and insertion of outer …

The guanine nucleotide exchange factor (GEF) Son of Sevenless (SOS) is a key Ras
activator that is autoinhibited in the cytosol and activates upon membrane recruitment …

Optical probes that provide information about local chirality have been developed based on
changes to the circular polarisation of emitted light. Highly emissive complexes of lanthanide …

The 3D organization of chromatin regulates many genome functions. Our understanding of
3D genome organization requires tools to directly visualize chromatin conformation in its …

Fluorescence provides a mechanism for achieving contrast in biological imaging that
enables investigations of molecular structure, dynamics, and function at high spatial and …

Single-molecule fluorescence spectroscopy and super-resolution microscopy are important
elements of the ongoing technical revolution to reveal biochemical and cellular processes in …