Engineering islets from stem cells for advanced therapies of diabetes

J Siehler, AK Blöchinger, M Meier… - Nature reviews Drug …, 2021 - nature.com
Diabetes mellitus is a metabolic disorder that affects more than 460 million people
worldwide. Type 1 diabetes (T1D) is caused by autoimmune destruction of β-cells, whereas …

Single-cell protein secretion detection and profiling

Z Chen, JJ Chen, R Fan - Annual Review of Analytical Chemistry, 2019 - annualreviews.org
Secreted proteins play important roles in mediating various biological processes such as
cell–cell communication, differentiation, migration, and homeostasis at the population or …

Analyzing immune response to engineered hydrogels by hierarchical clustering of inflammatory cell subsets

MA Fernandez-Yague, LA Hymel, CE Olingy… - Science …, 2022 - science.org
Understanding the immune response to hydrogel implantation is critical for the design of
immunomodulatory biomaterials. To study the progression of inflammation around poly …

Spatiotemporal NF-κB dynamics encodes the position, amplitude, and duration of local immune inputs

M Son, T Frank, T Holst-Hansen, AG Wang… - Science …, 2022 - science.org
Infected cells communicate through secreted signaling molecules like cytokines, which carry
information about pathogens. How differences in cytokine secretion affect inflammatory …

Identification of druggable regulators of cell secretion via a kinome-wide screen and high-throughput immunomagnetic cell sorting

M Labib, Z Wang, Y Kim, S Lin, A Abdrabou… - Nature Biomedical …, 2024 - nature.com
The identification of genetic regulators of cell secretions is challenging because it requires
the sorting of a large number of cells according to their secretion patterns. Here we report …

A mineralizing pool of Gli1-expressing progenitors builds the tendon enthesis and demonstrates therapeutic potential

F Fang, Y ** secretome-mediated interaction between paired neuron–macrophage single cells
J Deng, Y Ji, F Zhu, L Liu, L Li, X Bai… - Proceedings of the …, 2022 - National Acad Sciences
Neuron-immune interaction through secreted factors contributes significantly to the complex
microenvironment in the central nervous system that could alter cell functionalities and fates …

Spatial barcoding-enabled highly multiplexed immunoassay with digital microfluidics

H Li, X Liu, F Zhu, D Ma, C Miao, H Su, J Deng… - Biosensors and …, 2022 - Elsevier
Digital microfluidics (DMF), facilitating independent manipulation of microliter samples,
provides an ideal platform for immunoassay detection; however, suffering limited …

Multi‐Phenotypic Exosome Secretion Profiling Microfluidic Platform for Exploring Single‐Cell Heterogeneity

F Song, C Wang, C Wang, J Wang, Y Wu… - Small …, 2022 - Wiley Online Library
Cellular phenotypic and functional heterogeneities have advanced cancer evolution and
treatment resistance. Although exosome‐bound proteins reflect cellular functions, single …

Deciphering Cell–Cell Interactions with Integrative Single‐Cell Secretion Profiling

L Li, H Su, Y Ji, F Zhu, J Deng, X Bai, H Li… - Advanced …, 2023 - Wiley Online Library
Cell–cell interactions are the fundamental behaviors to regulate cellular activities. A
comprehensive evaluation of intercellular interactions requires direct profiling of various …