In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Stress granules (SGs) are cytosolic biomolecular condensates that form in response to
cellular stress. Weak, multivalent interactions between their protein and RNA constituents …

Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era.
We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data …

In the mitochondrial outer membrane, α-helical transmembrane proteins play critical roles in
cytoplasmic-mitochondrial communication. Using genome-wide CRISPR screens, we …

The ability to map trafficking for thousands of endogenous proteins at once in living cells
would reveal biology currently invisible to both microscopy and mass spectrometry. Here, we …

Protein self-assembly is one of the formation mechanisms of biomolecular condensates.
However, most phase-separating systems (PS) demand multiple partners in biological …

Astrocytes and neurons extensively interact in the brain. Identifying astrocyte and neuron
proteomes is essential for elucidating the protein networks that dictate their respective …

The lysosome is central to the degradation of proteins, carbohydrates, and lipids and their
salvage back to the cytosol for reutilization. Lysosomal transporters for amino acids, sugars …

Abstract Treatment options for COVID‐19, caused by SARS‐CoV‐2, remain limited.
Understanding viral pathogenesis at the molecular level is critical to develop effective …

The cell is a multi-scale structure with modular organization across at least four orders of
magnitude. Two central approaches for map** this structure—protein fluorescent imaging …