Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Biocatalysis has undergone revolutionary progress in the past century. Benefited by the
integration of multidisciplinary technologies, natural enzymatic reactions are constantly …

Metabolic engineers endeavor to create a bio-based manufacturing industry using microbes
to produce fuels, chemicals, and medicines. Plant natural products (PNPs) are historically …

Metabolic engineering of microorganisms to produce desirable products on an industrial
scale can result in unbalanced cellular metabolic networks that reduce productivity and …

Improvements in DNA synthesis and sequencing have underpinned comprehensive
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …

Engineering cellular metabolism for improved production of valuable chemicals requires
extensive modulation of bacterial genome to explore complex genetic spaces. Here, we …

In phenylketonuria (PKU) patients, a genetic defect in the enzyme phenylalanine
hydroxylase (PAH) leads to elevated systemic phenylalanine (Phe), which can result in …

Transcription-factor-based biosensors (TFBs) are often used for metabolite detection,
adaptive evolution, and metabolic flux control. However, designing TFBs with superior …

Genetic regulatory proteins inducible by small molecules are useful synthetic biology tools
as sensors and switches. Bacterial allosteric transcription factors (aTFs) are a major class of …

Biosensors are used to detect and quantify chemicals produced in industrial microbiology
with high specificity, sensitivity, and portability. Most biosensors, however, are limited by the …