Among various gene therapy methods for cancer, suicide gene therapy attracts a special
attention because it allows selective conversion of non-toxic compounds into cytotoxic drugs …

The adoptive transfer of T cells expressing chimeric antigen receptors (CARs) through
genetic engineering is one of the most promising new therapies for treating cancer patients …

The principle of using suicide genes for gene directed enzyme prodrug therapy (GDEPT) of
cancer has gained increasing significance during the 20 years since its inception. The astute …

Interactions between small molecules and proteins play critical roles in regulating and
facilitating diverse biological functions, yet our ability to accurately re-engineer the specificity …

Gene therapy of cancer offers the possibility of a targeted treatment that destroys tumors and
metastases, but not normal tissues. In gene-directed enzyme prodrug therapy (GDEPT), or …

The catalytic mechanism of Escherichia coli purine nucleoside phosphorylase (PNP) is
revised using site-directed mutagenesis, kinetic studies and structure determinations. The …

Nowadays, antimicrobial resistance is a serious concern associated with the reduced
efficacy of traditional antibiotics and an increased health burden worldwide. In response to …

Escherichia coli purine nucleoside phosphorylase (PNP) expressed in tumors converts
relatively nontoxic prodrugs into membrane-permeant cytotoxic compounds with high …

Engineering of enzymes to more efficiently activate genotoxic prodrugs holds great potential
for improving anticancer gene or antibody therapies. We report the development of a new …

Suicide gene therapy is an attractive strategy to selectively destroy cancer cells while
minimizing unnecessary toxicity to normal cells. Since this idea was first introduced more …