Microbial production of advanced biofuels
Concerns over climate change have necessitated a rethinking of our transportation
infrastructure. One possible alternative to carbon-polluting fossil fuels is biofuels produced …
infrastructure. One possible alternative to carbon-polluting fossil fuels is biofuels produced …
New applications of synthetic biology tools for cyanobacterial metabolic engineering
Cyanobacteria are promising microorganisms for sustainable biotechnologies, yet unlocking
their potential requires radical re-engineering and application of cutting-edge synthetic …
their potential requires radical re-engineering and application of cutting-edge synthetic …
Creation and analysis of biochemical constraint-based models using the COBRA Toolbox v. 3.0
Constraint-based reconstruction and analysis (COBRA) provides a molecular mechanistic
framework for integrative analysis of experimental molecular systems biology data and …
framework for integrative analysis of experimental molecular systems biology data and …
[HTML][HTML] Automating the design-build-test-learn cycle towards next-generation bacterial cell factories
Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …
technologies, developed over the past 20 years, have enormously accelerated the …
Recent advances in systems metabolic engineering tools and strategies
Highlights•Microbial cell factories are becoming important in sustainable chemical
production.•Systems metabolic engineering allows efficient microbial cell factory …
production.•Systems metabolic engineering allows efficient microbial cell factory …
Genome-scale metabolic rewiring improves titers rates and yields of the non-native product indigoidine at scale
High titer, rate, yield (TRY), and scalability are challenging metrics to achieve due to trade-
offs between carbon use for growth and production. To achieve these metrics, we take the …
offs between carbon use for growth and production. To achieve these metrics, we take the …
[HTML][HTML] A navigation guide of synthetic biology tools for Pseudomonas putida
Pseudomonas putida is a microbial chassis of huge potential for industrial and
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …
Model-based metabolic engineering enables high yield itaconic acid production by Escherichia coli
Itaconic acid is a high potential platform chemical which is currently industrially produced by
Aspergillus terreus. Heterologous production of itaconic acid with Escherichia coli could …
Aspergillus terreus. Heterologous production of itaconic acid with Escherichia coli could …
Automatic construction of metabolic models with enzyme constraints
Background In order to improve the accuracy of constraint-based metabolic models, several
approaches have been developed which intend to integrate additional biological …
approaches have been developed which intend to integrate additional biological …
[HTML][HTML] Model-guided dynamic control of essential metabolic nodes boosts acetyl-coenzyme A–dependent bioproduction in rewired Pseudomonas putida
Pseudomonas putida is evolutionarily endowed with features relevant for bioproduction,
especially under harsh operating conditions. The rich metabolic versatility of this species …
especially under harsh operating conditions. The rich metabolic versatility of this species …