Concerns over climate change have necessitated a rethinking of our transportation
infrastructure. One possible alternative to carbon-polluting fossil fuels is biofuels produced …

Cyanobacteria are promising microorganisms for sustainable biotechnologies, yet unlocking
their potential requires radical re-engineering and application of cutting-edge synthetic …

Constraint-based reconstruction and analysis (COBRA) provides a molecular mechanistic
framework for integrative analysis of experimental molecular systems biology data and …

Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …

Highlights•Microbial cell factories are becoming important in sustainable chemical
production.•Systems metabolic engineering allows efficient microbial cell factory …

Microbial bioengineering is a growing field for producing plant natural products (PNPs) in
recent decades, using heterologous metabolic pathways in host cells. Once heterologous …

Pseudomonas putida is a microbial chassis of huge potential for industrial and
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …

High titer, rate, yield (TRY), and scalability are challenging metrics to achieve due to trade-
offs between carbon use for growth and production. To achieve these metrics, we take the …

Itaconic acid is a high potential platform chemical which is currently industrially produced by
Aspergillus terreus. Heterologous production of itaconic acid with Escherichia coli could …

Computational modelling of metabolic networks has become an established procedure in
the metabolic engineering of production strains. One key principle that is frequently used to …