Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

JM Bolivar, JM Woodley… - Chemical Society …, 2022‏ - pubs.rsc.org
Enzyme immobilization has been develo** since the 1960s and although many industrial
biocatalytic processes use the technology to improve enzyme performance, still today we …

Parameters necessary to define an immobilized enzyme preparation

J Boudrant, JM Woodley, R Fernandez-Lafuente - Process Biochemistry, 2020‏ - Elsevier
Biocatalytic processes continue to find increasing application in industry. Therefore enzyme
immobilization has also become of increasing importance as a means of allowing enzyme …

Novozym 435: the “perfect” lipase immobilized biocatalyst?

C Ortiz, ML Ferreira, O Barbosa… - Catalysis Science & …, 2019‏ - pubs.rsc.org
Novozym 435 (N435) is a commercially available immobilized lipase produced by
Novozymes. It is based on immobilization via interfacial activation of lipase B from Candida …

On the taught new tricks of enzymes immobilization: An all-inclusive overview

RA Wahab, N Elias, F Abdullah, SK Ghoshal - Reactive and Functional …, 2020‏ - Elsevier
The primary means of immobilizing enzymes are to boost the enzyme productivity and
operational stability, alongside facilitating the reuse of enzymes. Notwithstanding the …

Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions

RC Rodrigues, JJ Virgen-Ortíz, JCS Dos Santos… - Biotechnology …, 2019‏ - Elsevier
Lipases are the most widely used enzymes in biocatalysis, and the most utilized method for
enzyme immobilization is using hydrophobic supports at low ionic strength. This method …

[HTML][HTML] Biocatalytic production of biolubricants: Strategies, problems and future trends

RRC Monteiro, Á Berenguer-Murcia… - Biotechnology …, 2023‏ - Elsevier
The increasing worries by the inadequate use of energy and the preservation of nature are
promoting an increasing interest in the production of biolubricants. After discussing the …

Modifying enzyme activity and selectivity by immobilization

RC Rodrigues, C Ortiz, Á Berenguer-Murcia… - Chemical society …, 2013‏ - pubs.rsc.org
Immobilization of enzymes may produce alterations in their observed activity, specificity or
selectivity. Although in many cases an impoverishment of the enzyme properties is observed …

Glutaraldehyde in bio-catalysts design: A useful crosslinker and a versatile tool in enzyme immobilization

O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres… - Rsc Advances, 2014‏ - pubs.rsc.org
Glutaraldehyde is one of the most widely used reagents in the design of biocatalysts. It is a
powerful crosslinker, able to react with itself, with the advantages that this may bring forth. In …

Different strategies for the lipase immobilization on the chitosan based supports and their applications

F Rafiee, M Rezaee - International Journal of Biological Macromolecules, 2021‏ - Elsevier
Immobilized enzymes have received incredible interests in industry, pharmaceuticals,
chemistry and biochemistry sectors due to their various advantages such as ease of …

Potential of different enzyme immobilization strategies to improve enzyme performance

C Garcia‐Galan, Á Berenguer‐Murcia… - Advanced Synthesis …, 2011‏ - Wiley Online Library
Enzyme biocatalysis plays a very relevant role in the development of many chemical
industries, eg, energy, food or fine chemistry. To achieve this goal, enzyme immobilization is …