FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
Single-molecule biophysics: at the interface of biology, physics and chemistry
Single-molecule methods have matured into powerful and popular tools to probe the
complex behaviour of biological molecules, due to their unique abilities to probe molecular …
complex behaviour of biological molecules, due to their unique abilities to probe molecular …
Visualizing the disordered nuclear transport machinery in situ
The approximately 120 MDa mammalian nuclear pore complex (NPC) acts as a gatekeeper
for the transport between the nucleus and cytosol. The central channel of the NPC is filled …
for the transport between the nucleus and cytosol. The central channel of the NPC is filled …
Single-molecule FRET unmasks structural subpopulations and crucial molecular events during FUS low-complexity domain phase separation
Biomolecular condensates formed via phase separation of proteins and nucleic acids are
thought to be associated with a wide range of cellular functions and dysfunctions. We dissect …
thought to be associated with a wide range of cellular functions and dysfunctions. We dissect …
Advanced time-correlated single photon counting techniques for spectroscopy and imaging in biomedical systems
W Becker, A Bergmann, GL Biscotti… - … Applications of Ultrafast …, 2004 - spiedigitallibrary.org
Time-correlated single photon counting (TCSPC) is based on the detection of single photons
of a periodic light signal, measurement of the detection time of the photons, and the build-up …
of a periodic light signal, measurement of the detection time of the photons, and the build-up …
Plasticity of an ultrafast interaction between nucleoporins and nuclear transport receptors
The mechanisms by which intrinsically disordered proteins engage in rapid and highly
selective binding is a subject of considerable interest and represents a central paradigm to …
selective binding is a subject of considerable interest and represents a central paradigm to …
Moderation of Arabidopsis root stemness by CLAVATA1 and ARABIDOPSIS CRINKLY4 receptor kinase complexes
Background The root system of higher plants originates from the activity of a root meristem,
which comprises a group of highly specialized and long-lasting stem cells. Their …
which comprises a group of highly specialized and long-lasting stem cells. Their …
PAM: a framework for integrated analysis of imaging, single-molecule, and ensemble fluorescence data
W Schrimpf, A Barth, J Hendrix, DC Lamb - Biophysical journal, 2018 - cell.com
Fluorescence microscopy and spectroscopy data hold a wealth of information on the
investigated molecules, structures, or organisms. Nowadays, the same fluorescence data set …
investigated molecules, structures, or organisms. Nowadays, the same fluorescence data set …
In vivo FRET–FLIM reveals cell-type-specific protein interactions in Arabidopsis roots
During multicellular development, specification of distinct cell fates is often regulated by the
same transcription factors operating differently in distinct cis-regulatory modules,,, either …
same transcription factors operating differently in distinct cis-regulatory modules,,, either …
Single-molecule protein folding: diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin inhibitor 2
We report single-molecule folding studies of a small, single-domain protein, chymotrypsin
inhibitor 2 (CI2). CI2 is an excellent model system for protein folding studies and has been …
inhibitor 2 (CI2). CI2 is an excellent model system for protein folding studies and has been …