Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial
resolution. We recently discovered that preserved biological specimens can be physically …

Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

SNT is an end-to-end framework for neuronal morphometry and whole-brain connectomics
that supports tracing, proof-editing, visualization, quantification and modeling of …

INTRODUCTION Neural circuits across the brain are composed of structures spanning
seven orders of magnitude in size that are assembled from thousands of distinct protein …

Importance Factors associated with synapse loss beyond amyloid-β plaques and
neurofibrillary tangles may more closely correlate with the emergence of cognitive deficits in …

Mutations in GBA1, the gene encoding the lysosomal enzyme β-glucocerebrosidase
(GCase), which cause Gaucher's disease, are the most frequent genetic risk factor for …

Fluorescence microscopy has become a ubiquitous method to observe the location of
specific molecular components within cells. However, the resolution of light microscopy is …

Although ubiquitous in biological studies, the enhanced green and yellow fluorescent
proteins (EGFP and EYFP) were not specifically optimized for neuroscience, and their …

Microglia, a resident CNS macrophage, are dynamic cells, constantly extending and
retracting their processes as they contact and functionally regulate neurons and other glial …

Resolving the distribution of specific proteins at the nanoscale in the ultrastructural context of
the cell is a major challenge in fluorescence microscopy. We report the discovery of a new …