Direct observation of autoubiquitination for an integral membrane ubiquitin ligase in ERAD

B Moochickal Assainar, K Ragunathan… - Nature …, 2024 - nature.com
The endoplasmic reticulum associated degradation (ERAD) pathway regulates protein
quality control at the endoplasmic reticulum. ERAD of lumenal and membrane proteins …

Motion screening of fiducial marker for improved localization precision and resolution in SMLM

K Li, J Ni, X Tan, Q Zhou, D Chen, B Cao, J Lin, T Lin… - Optics …, 2023 - opg.optica.org
Single-molecule localization microscopy (SMLM) provides unmatched high resolution but
relies on accurate drift correction due to the long acquisition time for each field of view. A …

[HTML][HTML] Dual-color Colocalization in Single-molecule Localization Microscopy to Determine the Oligomeric State of Proteins in the Plasma Membrane

HL Tan, S Bungert-Plümke, D Kortzak, C Fahlke… - Bio …, 2023 - ncbi.nlm.nih.gov
Determining the oligomeric state of membrane proteins is critical for understanding their
function. However, traditional ex situ methods like clear native gel electrophoresis can …

Determination of oligomeric organization of membrane proteins from native membranes at nanoscale-spatial and single-molecule resolution

G Walker, C Brown, X Ge, S Kumar, MD Muzumdar… - bioRxiv, 2023 - biorxiv.org
The oligomeric organization of membrane proteins in native cell membranes is a critical
regulator of their function. High-resolution quantitative measurements of oligomeric …

Structural characterisation of capsular transporter proteins from Neisseria meningitidis

L Masselot - 2023 - research-repository.uwa.edu.au
Neisseria meningitidis (Nm) uses a polysaccharide capsule to protect itself and escape the
immune system. Polysaccharide transporters are complexes inserted into bacterial …

Oligomerization and cellular localization of SLC26A11

S Bungert-Pluemke, RE Guzman, C Fahlke - bioRxiv, 2024 - biorxiv.org
The solute carrier family 26 (SLC26) encompasses multifunctional anion exchangers in all
kingdoms of life. SLC26 proteins are known to assemble as dimers, and co-expression of …

Bitesize bundles F-actin and influences actin remodeling in syncytial Drosophila embryo development

AR Yeh, GJ Hoeprich, BL Goode, AC Martin - bioRxiv, 2023 - biorxiv.org
Actin networks undergo rearrangements that influence cell and tissue shape. Actin network
assembly and organization is regulated in space and time by a host of actin binding …