A layer of mucus covers the surface of all wet epithelia throughout the human body. Mucus is
a hydrogel mainly composed of water, mucins (glycoproteins), DNA, proteins, lipids, and cell …

Polymeric vehicles are versatile tools for therapeutic gene delivery. Many polymers—when
assembled with nucleic acids into vehicles—can protect the cargo from degradation and …

Chitosans are versatile biopolymers with multiple biological activities and potential
applications. They are linear copolymers of glucosamine and N-acetylglucosamine defined …

Coacervation is a liquid–liquid phase separation that can occur in solutions of
macromolecules through self-assembly or electrostatic interactions. Recently, coacervates …

A facile method was designed that can specifically deliver CRISPR/Cas9 into target cells
nuclei and reduce the off-target effects. A multifunctional delivery vector for FOXM1 knockout …

Hydrophobized chitosan derivatives, hexyl chitosan (HCS), dodecyl chitosan (DCS), and
phthaloyl chitosan (PhCS) of approximately 30 and 50% degree of substitution (% DS) …

Non-viral gene delivery vectors have lagged far behind viral ones in the current pipeline of
clinical trials of gene therapy nanomedicines. Even when non-viral nanovectors pose less …

Chitosan-loaded nanomedicines provide a greater opportunity for the treatment of
respiratory diseases. Natural biopolymer chitosan and its derivatives have a large number of …

Genetic and rare diseases (GARDs) affect more than 350 million patients worldwide and
remain a significant challenge in the clinic. Hence, continuous efforts have been made to …

Chitosan has been widely employed to deliver nucleic acids such as siRNA and plasmids.
However, chitosan-mediated delivery of a gene-editing system has not been reported yet. In …