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Analytical methods for process and product characterization of recombinant adeno-associated virus-based gene therapies
The optimization of upstream and downstream processes for production of recombinant
adeno-associated virus (rAAV) with consistent quality depends on the ability to rapidly …
adeno-associated virus (rAAV) with consistent quality depends on the ability to rapidly …
Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently
The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …
ability to modify the sequence, and hence the structure and activity, of individual proteins in …
Forensic trace DNA: a review
DNA analysis is frequently used to acquire information from biological material to aid
enquiries associated with criminal offences, disaster victim identification and missing …
enquiries associated with criminal offences, disaster victim identification and missing …
Application of flow cytometry to industrial microbial bioprocesses
Flow cytometry (FC) is a powerful technique for the rapid analysis of single cells in a mixture,
by means of light-scattering and fluorescence measurements. In biotechnology, the power of …
by means of light-scattering and fluorescence measurements. In biotechnology, the power of …
Past, present and future applications of flow cytometry in aquatic microbiology
Flow cytometry (FCM) is rapidly becoming an essential tool in the field of aquatic
microbiology. It provides opportunities for microbial analysis at both the community and …
microbiology. It provides opportunities for microbial analysis at both the community and …
Evaluating metabolic stress and plasmid stability in plasmid DNA production by Escherichia coli
In the context of recombinant DNA technology, the development of feasible and high-
yielding plasmid DNA production processes has regained attention as more evidence for its …
yielding plasmid DNA production processes has regained attention as more evidence for its …
Cytometric methods for measuring bacteria in water: advantages, pitfalls and applications
Rapid detection of microbial cells is a challenge in microbiology, particularly when complex
indigenous communities or subpopulations varying in viability, activity and physiological …
indigenous communities or subpopulations varying in viability, activity and physiological …
Guidelines to cell engineering for monoclonal antibody production
Monoclonal antibodies (mAbs) are currently used for many diagnostic and therapeutic
applications. The high demand for these biopharmaceuticals has led to the development of …
applications. The high demand for these biopharmaceuticals has led to the development of …
Extracellular vesicles as a platform for 'liquid biopsy'in glioblastoma patients
Extracellular vesicles (EVs) are cell-secreted vesicles that range from 30–2000 nm in size.
These vesicles are secreted by both normal and neoplastic cells. Physiologically, EVs serve …
These vesicles are secreted by both normal and neoplastic cells. Physiologically, EVs serve …
Electrokinetically driven micro flow cytometers with integrated fiber optics for on-line cell/particle detection
This paper presents an innovative micro flow cytometer which is capable of counting and
sorting cells or particles. This compact device employs electrokinetic forces rather than the …
sorting cells or particles. This compact device employs electrokinetic forces rather than the …