Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

The promise of single-objective light-sheet microscopy is to combine the convenience of
standard single-objective microscopes with the speed, coverage, resolution and gentleness …

High-throughput methods for slide-free three-dimensional (3D) pathological analyses of
whole biopsies and surgical specimens offer the promise of modernizing traditional …

The contrast and resolution of images obtained with optical microscopes can be improved
by deconvolution and computational fusion of multiple views of the same sample, but these …

The limited per-pixel bandwidth of most microscopy methods requires compromises
between field of view, sampling density and imaging speed. This limitation constrains …

Recently developed approaches for highly multiplexed imaging have revealed complex
patterns of cellular positioning and cell-cell interactions with important roles in both cellular …

Prostate cancer treatment planning is largely dependent upon examination of core-needle
biopsies. The microscopic architecture of the prostate glands forms the basis for prognostic …

Tissue clearing has become a powerful technique for studying anatomy and morphology at
scales ranging from entire organisms to subcellular features. With the recent proliferation of …

In 2015, we launched the mesoSPIM initiative, an open-source project for making light-sheet
microscopy of large cleared tissues more accessible. Meanwhile, the demand for imaging …