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Top-down proteomics
Proteoforms, which arise from post-translational modifications, genetic polymorphisms and
RNA splice variants, play a pivotal role as drivers in biology. Understanding proteoforms is …
RNA splice variants, play a pivotal role as drivers in biology. Understanding proteoforms is …
Top-down proteomics and the challenges of true proteoform characterization
Top-down proteomics (TDP) aims to identify and profile intact protein forms (proteoforms)
extracted from biological samples. True proteoform characterization requires that both the …
extracted from biological samples. True proteoform characterization requires that both the …
The Blood Proteoform Atlas: A reference map of proteoforms in human hematopoietic cells
Human biology is tightly linked to proteins, yet most measurements do not precisely
determine alternatively spliced sequences or posttranslational modifications. Here, we …
determine alternatively spliced sequences or posttranslational modifications. Here, we …
The human proteoform project: defining the human proteome
Proteins are the primary effectors of function in biology, and thus, complete knowledge of
their structure and properties is fundamental to deciphering function in basic and …
their structure and properties is fundamental to deciphering function in basic and …
Overview and considerations in bottom-up proteomics
Proteins are the key biological actors within cells, driving many biological processes integral
to both healthy and diseased states. Understanding the depth of complexity represented …
to both healthy and diseased states. Understanding the depth of complexity represented …
Top-down mass spectrometry of native proteoforms and their complexes: a community study
The combination of native electrospray ionization with top-down fragmentation in mass
spectrometry (MS) allows simultaneous determination of the stoichiometry of noncovalent …
spectrometry (MS) allows simultaneous determination of the stoichiometry of noncovalent …
Mass spectrometry-based top-down proteomics in nanomedicine: proteoform-specific measurement of protein corona
Conventional mass spectrometry (MS)-based bottom-up proteomics (BUP) analysis of the
protein corona [ie, an evolving layer of biomolecules, mostly proteins, formed on the surface …
protein corona [ie, an evolving layer of biomolecules, mostly proteins, formed on the surface …
O-Pair Search with MetaMorpheus for O-glycopeptide characterization
Abstract We report O-Pair Search, an approach to identify O-glycopeptides and localize O-
glycosites. Using paired collision-and electron-based dissociation spectra, O-Pair Search …
glycosites. Using paired collision-and electron-based dissociation spectra, O-Pair Search …
Ion activation methods for peptides and proteins
Mass spectrometry is well-established as one of the primary methods used to characterize
peptides and proteins. New advances in instrumentation and innovative analytical workflows …
peptides and proteins. New advances in instrumentation and innovative analytical workflows …
MASH explorer: a universal software environment for top-down proteomics
Top-down mass spectrometry (MS)-based proteomics enable a comprehensive analysis of
proteoforms with molecular specificity to achieve a proteome-wide understanding of protein …
proteoforms with molecular specificity to achieve a proteome-wide understanding of protein …